Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil

Authors

  • Thalita PPS Cruz Laboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, Brazil https://orcid.org/0000-0002-0167-6710
  • Francisco KSF Azevedo Department of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil
  • Marco A Pepato Department of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil https://orcid.org/0000-0002-7896-6329
  • Paula S Rizzo Department of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil https://orcid.org/0000-0003-0934-5139
  • Francisco JD Souto Department of Internal Medicine, Federal University of Mato Grosso, Cuiabá, MT, Brazil
  • Bertram Brenig Institute of Veterinary Medicine, University of Göttingen, Göttingen, Niedersachsen, Germany https://orcid.org/0000-0002-7635-9656
  • Aristóteles Góes-Neto Laboratory of Molecular Computational Biology of Fungi (LBMCF), Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
  • Siomar C Soares Department of Microbiology, Immunology, and Parasitology, Federal University of Triangle Miner, Uberaba, Brazil https://orcid.org/0000-0001-7299-3724
  • Rommel TJ Ramos Center of Genomics and Systems Biology, Institute of Biological Sciences, Federal University of Pará (UFPA), Belém, Pará, Brazil
  • Vasco AC Azevedo Laboratory of Cellular and Molecular Genetics (LGCM), Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
  • Mateus M Costa Laboratory of Microbiology and Animal Immunology, Federal University of Vale of São Francisco (Univasf), Petrolina, Pernambuco, Brazil - Center for Open Access Genomic Analysis (CALAnGO), Federal University of Vale of São Francisco (Univasf), Petrolina, Pernambuco, Brazil
  • Leonardo Broetto Multicentric Graduate Program in Biochemistry and Molecular Biology at SBBq (PMBqBM), Federal University of Alagoas, Brazil https://orcid.org/0000-0002-5424-2115
  • Luciano Nakazato Laboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, Brazil
  • Valéria Dutra Laboratory of Veterinary Microbiology and Molecular Biology, Veterinary Hospital of Federal University of Mato Grosso, Cuiabá, MT, Brazil

DOI:

https://doi.org/10.3855/jidc.18913

Keywords:

Serratia marcescens, outbreak, antimicrobial resistance, genomic sequencing

Abstract

Introduction: Serratia marcescens is an opportunistic pathogen found ubiquitously in the environment and associated with a wide range of nosocomial infections. This multidrug-resistant bacterium has been a cause of concern for hospitals and healthcare facilities due to its ability to spread rapidly and cause outbreaks. Next generation sequencing genotyping of bacterial isolates has proven to be a valuable tool for tracking the spread and transmission of nosocomial infections. This has allowed for the identification of outbreaks and transmission chains, as well as determining whether cases are due to endogenous or exogenous sources. Evidence of nosocomial transmission has been gathered through genotyping methods. The aim of this study was to investigate the genetic diversity of carbapenemase-producing S. marcescens in an outbreak at a public hospital in Cuiaba, MT, Brazil.

Methodology: Ten isolates of S. marcenses were sequenced and antibiotic resistance profiles analyzed over 12 days.

Results: The isolates were clonal and multidrug resistant. Gentamycin and tigecycline had sensitivity in 90% and 80% isolates, respectively. Genomic analysis identified several genes that encode β-lactamases, aminoglycoside-modifying enzymes, efflux pumps, and other virulence factors.

Conclusions: Systematic surveillance is crucial in monitoring the evolution of S. marcescens genotypes, as it can lead to early detection and prevention of outbreaks.

Downloads

Published

2024-05-30

How to Cite

1.
Cruz TP, Azevedo FK, Pepato MA, Rizzo PS, Souto FJ, Brenig B, Góes-Neto A, Soares SC, Ramos RT, Azevedo VA, Costa MM, Broetto L, Nakazato L, Dutra V (2024) Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil. J Infect Dev Ctries 18:726–731. doi: 10.3855/jidc.18913

Issue

Vol. 18 No. 05: May 2024

Section

Original Articles

License

Copyright (c) 2024 Thalita Priscila Peres Seabra da Cruz, Francisco Kennedy Scofoni Faleiros de Azevedo, Marco Andrey Pepato, Paula Sossai Rizzo, Francisco Souto, Bertram Brenig, Aristóteles Góes-Neto, Siomar Castro Soares, Rommel Thiago Jucá Ramos, Vasco Ariston de Carvalho Azevedo, Mateus Matiuzzi da Costa , Leonardo Broetto, Luciano Nakazato, Valéria Dutra

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Crossref
Scopus
Google Scholar
Europe PMC