Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae

Boukaré Kaboré; Ganamé A Ouédraogo; Henri S Ouédraogo; Hama Cissé; Oumarou Zongo; Koudbi J Zongo; Boukaré Zeba; Idrissa Sanou; Aly Savadogo

doi:10.3855/jidc.17519

Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae

Authors

Boukaré Kaboré Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021
Ganamé A Ouédraogo Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021
Henri S Ouédraogo Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021
Hama Cissé Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021
Oumarou Zongo Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021
Koudbi J Zongo Department of Biochemistry and Microbiology, Faculty of Applied Science and Technology, University of Dédougou, Burkina Faso BP 176
Boukaré Zeba Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021
Idrissa Sanou UFR Health Sciences, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso, 03 BP 7021
Aly Savadogo Department of Biochemistry and Microbiology, Laboratory of Applied Biochemistry and Immunology, University Joseph KI-ZERBO, Ouagadougou 03, Burkina Faso 03 BP 7021

DOI:

https://doi.org/10.3855/jidc.17519

Keywords:

Klebsiella pneumoniae, carbapenemases, (GTG)5-PCR

Abstract

Introduction: Klebsiella pneumoniae is an encapsulated Gram-negative bacterium that is responsible for numerous infections in healthcare facilities worldwide and is frequently isolated. The World Health Organization has listed K. pneumoniaeas as a critical antibiotic resistant bacterial pathogen for which new antibiotics are urgently needed. This study aimed to use molecular tools to identify and examine antibiotic resistance in clinical strains of K. pneumoniae.

Methodology: A total of 15 unduplicated K. pneumoniae strains isolated from patient samples with multidrug-resistant (MDR) profiles were subjected to polymerase chain reaction (PCR) to amplify the most common carbapenem resistance genes. (GTG)5 PCR and phylogenetic analysis were performed to identify the genetic relationship between the strains.

Results: All strains yielded a (GTG)5-PCR profile, and this allowed us to group these strains into 8 groups according to the size and number of characteristic bands. Phylogenetic analysis was done using the free software UPGMA and a single bacterial clone with a correlation coefficient of over 97% was identified. New Delhi metallo-beta-lactamase NDM-like (blaNDM) carbapenem resistance genes were detected in three strains of K. pneumoniae, which represented a resistance rate of 20%. However, carbapenemases type A [Klebsiella pneumoniae carbapenemase (KPC) and imipenem-hydrolysing beta-lactamase (IMI), type D [oxacillinase-48 (OXA-48)], and other metallo-β-lactamase [Verona integron-encoded metallo-beta-lactamase (VIM), and enzyme active on imipenem (IMP)] were not detected.

Conclusions: We identified and grouped the blaNDM resistance genes of Klebsiella pneumonia strains.

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Published

2024-09-30

How to Cite

Kaboré B, Ouédraogo GA, Ouédraogo HS, Cissé H, Zongo O, Zongo KJ, Zeba B, Sanou I, Savadogo A (2024) Identification and phylogenetic analysis of carbapenemase genes from clinical strains of Klebsiella pneumoniae. J Infect Dev Ctries 18:1387–1393. doi: 10.3855/jidc.17519

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Issue

Vol. 18 No. 09: September 2024

Section

Original Articles

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