Colistin and β-lactam resistance in Escherichia coli isolates from bovines, swine, and humans

Authors

  • Nayla Kellen de O Ventura Universidade Federal de Viçosa (UFV), Departamento de Veterinária, InsPOA — Laboratório de Inspeção de Produtos de Origem Animal, Viçosa, Minas Gerais, Brazil https://orcid.org/0000-0003-3167-0712
  • Leslye R Freitas Universidade Federal de Viçosa (UFV), Departamento de Veterinária, InsPOA — Laboratório de Inspeção de Produtos de Origem Animal, Viçosa, Minas Gerais, Brazil https://orcid.org/0009-0001-7823-307X
  • Flaviana A Sousa Universidade Federal de Viçosa (UFV), Departamento de Veterinária, InsPOA — Laboratório de Inspeção de Produtos de Origem Animal, Viçosa, Minas Gerais, Brazil https://orcid.org/0000-0002-4413-8357
  • Marcus Vinicius C Cossi Universidade Federal de Uberlândia (UFU), Departamento de Veterinária, Uberlândia, Minas Gerais, Brazil https://orcid.org/0000-0002-5744-3510
  • Luis Augusto Nero Universidade Federal de Viçosa (UFV), Departamento de Veterinária, InsPOA — Laboratório de Inspeção de Produtos de Origem Animal, Viçosa, Minas Gerais, Brazil
  • Ricardo S Yamatogi Universidade Federal de Viçosa (UFV), Departamento de Veterinária, InsPOA — Laboratório de Inspeção de Produtos de Origem Animal, Viçosa, Minas Gerais, Brazil https://orcid.org/0000-0002-0068-4217

DOI:

https://doi.org/10.3855/jidc.20143

Keywords:

bla, mcr, pmrAB, resistance

Abstract

Introduction: Colistin and β-lactams are widely investigated because of their effectiveness in the treatment of human diseases. This study investigated the phenotypic and genotypic profiles of colistin- and β-lactam-resistant Escherichia coli (n = 235) obtained from bovines, swine, and workers from a mixed slaughterhouse in Brazil.

Methodology: The disk diffusion method was used to test the resistance against β-lactams (amoxicillin, ampicillin, cefaclor, cefazolin, cefepime, cefotaxime, ceftazidime, ceftriaxone, imipenem, meropenem, and aztreonam). In order to test colistin resistance, the isolates were subjected to the minimum inhibitory concentration (MIC) technique using the broth microdilution method (BMD; 0.5 to 16 μg/mL) and polymerase chain reaction (PCR) assays targeting colistin- (mcr-1 to mcr-5) and β-lactam- (blaTEM, blaCTX-M, blaSHV, ampC) genes. The pmrAB mutation was further investigated.

Results: The isolates presented resistance, especially to ampicillin (cattle: 14/106, swine: 62/100, humans: 10/29) and amoxicillin (cattle: 7/106, swine: 61/100, humans: 8/29). One swine isolate was characterized as extended spectrum β-lactamase (ESBL) producer. The isolates obtained from swine presented higher frequencies of colistin resistance (13/100) when compared to isolates from bovines (5/106) and humans (0/29). Molecular assays concluded that the isolates presented blaTEM (swine: 67/100, humans: 7/29), ampC (swine: 1/100), and blaCTXM (swine: 1/100). The pmrAB complex presented mutations (T31S, P42A, I128N, G144S, H2R, N358Y, D283G, K15I).

Conclusions: This study highlights the presence of antimicrobial resistance and presents a method to verify these factors in the animal production chain.

Downloads

Published

2025-01-31

How to Cite

1.
Ventura NK de O, Freitas LR, Sousa FA, Cossi MVC, Nero LA, Yamatogi RS (2025) Colistin and β-lactam resistance in Escherichia coli isolates from bovines, swine, and humans. J Infect Dev Ctries 19:49–57. doi: 10.3855/jidc.20143

Issue

Vol. 19 No. 01: January 2025

Section

Original Articles

License

Copyright (c) 2025 Nayla Kellen de Oliveira Ventura, Leslye Rocha Freitas, Flaviana Antunes Sousa, Marcus Vinicius Coutinho Cossi, Luis Augusto Nero, Ricardo Seiti Yamatogi

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Crossref
Scopus
Google Scholar
Europe PMC