Analysis of diarrheagenic potential of uropathogenic Escherichia coli isolates in Dhaka, Bangladesh

  • Marium Khaleque University of Dhaka, Dhaka, Bangladesh
  • Selina Akter Jessore University of Science and Technology, Jessore, Bangladesh
  • Humaira Akhter University of Dhaka, Dhaka, Bangladesh
  • Sirajul Islam Khan University of Dhaka, Dhaka, Bangladesh
  • Anowara Begum University of Dhaka, Dhaka, Bangladesh

Abstract

Abstract


Introduction: Urinary tract infection is the most frequently diagnosed kidney and urologic disease.


Methodology: Whether the Escherichia coli strains responsible for urinary tract infection (UPEC) carry virulence properties of diarrheagenic E. coli (DEC), 56 UPEC strains were examined for the presence of DEC and UPEC characteristics (e.g. biofilm formation, hemolysis activity, virulence genes).


Results: Among 56 UPEC strains, 21 showed capable of biofilm formation and only 5 showed hemolysis activity on sheep blood agar. In Multiplex PCR on assessment of virulence genes related to uropathogenesis; 42% was found positive for papC gene, 27% was fim1 positive, 11% was afa positive and none was found positive for sfa. Most of the isolates were found carrying none of eight diarrhea associated genes (e.g. estA, eltB, vt1, vt2, eaeA, ea, ial and bfpA) as expected. Only seven isolates were found to harbor these genes: five genes i.e., vt2, ial, eltB, bfpA and ea were found in five different isolates and two isolates were positive for estA, among these two, one was found positive for fim1, papC along with estA, a UPEC strain containing virulent gene of ETEC strain. One isolate was found carrying fim1 and vt2 showing the property of EHEC and another isolate was found positive for fim1 and ial, the characteristic of EIEC. One isolate harboring bfpA gene characterized as EPEC and the another one was found to harbor ea gene, characterized as EAEC.


Conclusions: This study observed that most UPEC strains are unique to uropathogenesis, still very few may carry the diarrheagenic property.


 

Author Biographies

Marium Khaleque, University of Dhaka, Dhaka, Bangladesh

Research Fellow,

Department of Microbiology,
University of Dhaka

Dhaka-1000

Bangladesh

Selina Akter, Jessore University of Science and Technology, Jessore, Bangladesh

Asisstant Professor,

Department of Microbiology,
Jessore University of Science and Technology

Jessore,

Bangladesh

 

Humaira Akhter, University of Dhaka, Dhaka, Bangladesh

Professor ,

Department of Microbiology,
University of Dhaka

Dhaka-1000

Bangladesh

Sirajul Islam Khan, University of Dhaka, Dhaka, Bangladesh

Professor ,

Department of Microbiology,

University of Dhaka

Dhaka-1000

Bangladesh

Anowara Begum, University of Dhaka, Dhaka, Bangladesh

Professor ,

Department of Microbiology,

University of Dhaka

Dhaka-1000

Bangladesh

Published
2017-06-24
How to Cite
Khaleque M, Akter S, Akhter H, Khan S, Begum A (2017) Analysis of diarrheagenic potential of uropathogenic Escherichia coli isolates in Dhaka, Bangladesh. The Journal Of Infection In Developing Countries 11 (06): 459-469. https://doi.org/10.3855/jidc.8257
Section
Original Articles

Keywords

Key word: Diarrheagenic E. coli; ETEC; Uropathogenic E. coli; UTI; virulence genes