Antimicrobial resistance and integrons of ESBL-producing thermotolerant coliforms from a water reservoir in Tai'an, China

  • Hongna Zhang Hebei University of Ecomonics and Business, Shijiazhuang, China
  • Xiaonan Zhao Shandong Agricultural University, Tai'an, China
  • Zhenzhen Zhai Central Hospital of Tai'an City, Tai'an, China
  • Qing Li Shandong Agricultural University, Tai'an, China
  • Shuyuan Guo Shandong Agricultural University, Tai'an, China
  • Weishan Chang Shandong Agricultural University, Tai'an, China

Abstract

Introduction: The contamination of water environments by extended-spectrum β-lactamases-producing thermotolerant coliforms (ESBL-TC) has aroused public concern. However, little epidemiological data on ESBL-TC isolates from water reservoirs is available in China.


Methodology: This study was designed to investigate antibiotic resistance, bla gene types, and the presence of integrons (class 1, 2, and 3) and gene cassettes in ESBL-TC isolated from the Huangqian Reservoir of Tai'an, China.


Results: A total of 96 non-duplicate ESBL-TC were obtained in this study and the ESBL genes included blaCTX-M-14 (n = 47), blaCTX-M-15 (n = 27), blaCTX-M-55 (n = 18), blaSHV-12 (n = 4), blaCTX-M-3 (n = 3), and blaCTX-M-123 (n = 1). Eighty-three of the ninety-six ESBL-TC contained class 1 integrons (86.5%), and 2 isolates harbored class 2 integrons. The sizes of gene cassette regions within integrons were ranged from 0.2 kb to 3.2 kb.


Conclusions: The findings of this study indicated the widespread presence of ESBL-TC strains in the Huangqian Reservoir and spotlighted the potential role of water bodies as reservoirs for antibiotic resistant genes.

Author Biographies

Hongna Zhang, Hebei University of Ecomonics and Business, Shijiazhuang, China

Department of Teaching Management

 

Xiaonan Zhao, Shandong Agricultural University, Tai'an, China

College of Animal Science and Technology

Zhenzhen Zhai, Central Hospital of Tai'an City, Tai'an, China

Digestive Disease Institute

Qing Li, Shandong Agricultural University, Tai'an, China

College of Animal Science and Technology

Shuyuan Guo, Shandong Agricultural University, Tai'an, China

College of Animal Science and Technology

Weishan Chang, Shandong Agricultural University, Tai'an, China

College of Animal Science and Technology

Published
2017-11-06
How to Cite
Zhang H, Zhao X, Zhai Z, Li Q, Guo S, Chang W (2017) Antimicrobial resistance and integrons of ESBL-producing thermotolerant coliforms from a water reservoir in Tai'an, China. The Journal Of Infection In Developing Countries 11 (10): 740-746. https://doi.org/10.3855/jidc.8449
Section
Original Articles