Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia

  • Hajer Kilani University of Tunis El Manar, Bab Saadoun, Tunis, Tunisia
  • Mohamed Salah Abbassi University of Tunis El Manar, Bab Saadoun, Tunis, Tunisia
  • Sana Ferjani Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia
  • Rakia Ben Salem University of Tunis El Manar, Bab Saadoun, Tunis, Tunisia
  • Riadh Mansouri Emergency Center for Transboundary Animal Diseases (FAOSNE), Tunis, Tunisia
  • Noureddine Ben Chehida University of Tunis El Manar, Bab Saadoun, Tunis, Tunisia
  • Ilhem Boutiba-Ben Boubaker Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia

Abstract

Introduction: The virulent Escherichia coli strains responsible for extraintestinal infections were mainly belonged to B2 and D phylogroups. However, no past studies have determinate via the presence of virulence genes the frequency of E. coli pathovars recovered from animals housed in farms in Tunisia. The aims of this study were to investigate 26 E. coli isolated from healthy and diarrheic animals and to determinate via the presence of virulence genes the frequency of pathovars.


Methodology: Twenty-six E. coli isolates of phylogroups B2 (n = 14), B22 (n = 9), B23 (n = 5), and D2 (n = 12) were characterized. Genes encoding virulence factors (fimH,eaeA,aggC,papC, papG allele III, hlyA, east1, cnf1, exhA,stx1, stx2, iutA, fyuA, ibeA,and ipaH), and antibiotic resistance as well as class 1 and 2 integrons were searched by polymerase chain reaction (PCR). The genetic relationship of isolates was done by PFGE.


Results: According to the occurrence of specific genes the 26 isolates were classified as:9 EAEC, 2 EHEC, 4 UPEC, 3 EPEC/EHEC and 1 NTEC. Therefore, 2 Ex-PEC and 5 APEC were presented amongst our strains. Some isolates (12) were clonal and the remaining was unrelated.


Conclusions: Higher diversity of pathovars which carried diverse combinations of virulence genes in healthy isolates. In addition, it seems that the infections were caused by different mechanisms.

Author Biographies

Hajer Kilani, University of Tunis El Manar, Bab Saadoun, Tunis, Tunisia

1-      University of  Tunis El Manar, Veterinary Research Institute of Tunisia, Bab Saadoun, Tunis, Tunisia

2-      University of Tunis El Manar, Faculty of Medecin of Tunis, LR99ES09 Laboratory of Antibiotic resistance, Tunis, Tunisia

 

Mohamed Salah Abbassi, University of Tunis El Manar, Bab Saadoun, Tunis, Tunisia

1-      University of  Tunis El Manar, Veterinary Research Institute of Tunisia, Bab Saadoun, Tunis, Tunisia

2-      University of Tunis El Manar, Faculty of Medecin of Tunis, LR99ES09 Laboratory of Antibiotic resistance, Tunis, Tunisia

Sana Ferjani, Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia

1-      University of Tunis El Manar, Faculty of Medicin of Tunis, LR99ES09 Laboratory of Antibiotic resistance, Tunis, Tunisia

2-      Hospital of Charles Nicolle, Department of Microbiology, Tunis,Tunisia

 

Rakia Ben Salem, University of Tunis El Manar, Bab Saadoun, Tunis, Tunisia

1-      University of  Tunis El Manar, Veterinary Research Institute of Tunisia, Bab Saadoun, Tunis, Tunisia

 

Riadh Mansouri, Emergency Center for Transboundary Animal Diseases (FAOSNE), Tunis, Tunisia

4- Emergency Center for Transboundary Animal Diseases (FAOSNE)

Noureddine Ben Chehida, University of Tunis El Manar, Bab Saadoun, Tunis, Tunisia

1-      University of  Tunis El Manar, Veterinary Research Institute of Tunisia, Bab Saadoun, Tunis, Tunisia

 

Ilhem Boutiba-Ben Boubaker, Faculty of Medicine, University of Tunis El Manar, Tunis, Tunisia

1-      University of Tunis El Manar, Faculty of Medecin of Tunis, LR99ES09 Laboratory of Antibiotic resistance, Tunis, Tunisia

2-      Hospital of Charles Nicolle, Department of Microbiology, Tunis,Tunisia

 

 

Published
2017-07-31
How to Cite
Kilani H, Abbassi M, Ferjani S, Ben Salem R, Mansouri R, Ben Chehida N, Boutiba-Ben Boubaker I (2017) Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia. The Journal Of Infection In Developing Countries 11 (07): 549-556. https://doi.org/10.3855/jidc.8579
Section
Original Articles

Keywords

Escherichia coli, virulence genes, genetic diversity, pathovars