Drug resistance pattern and clonality in H. pylori strains

Varsha Singh; Shrutkirti Mishra; Pushpa Maurya; GRKoteswar Rao; Ashok Kumar Jain; Vinod Kumar Dixit; Anil Kumar Gulati; Gopal Nath

doi:10.3855/jidc.60

Drug resistance pattern and clonality in H. pylori strains

Authors

Varsha Singh Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi
Shrutkirti Mishra Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi
Pushpa Maurya Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi
GRKoteswar Rao Department of Biochemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi
Ashok Kumar Jain Department of Gastroenterology, Institute of Medical Sciences, Banaras Hindu University, Varanasi
Vinod Kumar Dixit Department of Gastroenterology, Institute of Medical Sciences, Banaras Hindu University, Varanasi
Anil Kumar Gulati Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi
Gopal Nath Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi

DOI:

https://doi.org/10.3855/jidc.60

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title

Abstract

Background: This aim of this work was to determine the in vitro activity of clarithromycin, amoxycillin, metronidazole and tetracycline against Helicobacter pylori and clonality among resistant and sensitive strains isolated from North India. Methodology: A total of 68 H. pylori isolates from peptic ulcer disease and non ulcer dyspepsia patients were examined. These strains were subjected for determination of minimum inhibitory concentration of clarithromycin, amoxycillin, metronidazole and tetracycline. For molecular characterization of resistant and sensitive strains, enterobacterial repetitive intergenic consensus sequences (ERIC) and random amplified polymorphic DNA-PCR (RAPD-PCR) methods were used. Results: All the tested isolates were found resistant to metronidazole, while 65% were resistant to amoxycillin and 4.7% were resistant to clarithromycin. However, none of the isolates were found to be resistant to tetracycline. Molecular fingerprinting and cluster analysis of resistant and sensitive strains did not give clues for clonal spread of resistant strains. Conclusions: Various chromosomal mutations were seen in the putative resistance genes of resistant strains, possibly indicating selection pressure as the major cause of high resistance.

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Published

2009-03-01

How to Cite

Singh V, Mishra S, Maurya P, Rao G, Jain AK, Dixit VK, Gulati AK, Nath G (2009) Drug resistance pattern and clonality in H. pylori strains. J Infect Dev Ctries 3:130–136. doi: 10.3855/jidc.60

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Issue

Vol. 3 No. 02: March 2009

Section

Brief Original Articles

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