Genotyping of Mycobacterium tuberculosis in Lebanon using a novel rapid spoligotyping multiplex luminex method

  • Khaldoun Massoud American University of Beirut Medical Center
  • George F Araj American University of Beirut Medical Center
  • Sukayna Fadlallah American University of Beirut
  • Lina Reslan American University of Beirut Medical Center
  • Lina Itani American University of Beirut Medical Center
  • Aline Avedissian American University of Beirut Medical Center
  • Ghassan Dbaibo American University of Beirut Medial Center
  • Antoine Saade Lebanese Anti-Tuberculosis Association
  • Ghassan M Matar American University of Beirut
Keywords: Tuberculosis, Spoligotyping, Luminex

Abstract

Introduction: Incidence of Tuberculosis (TB) in Lebanon, according to the WHO, is estimated to be 35 cases per 100,000 people. However, data about the genotypes of circulating Mycobacterium tuberculosis isolates (MTB) in this country is lacking. This study aims to reveal the genotypes of TB isolates recovered from patients in Lebanon.

Methodology: Fifty M. tuberculosis isolates from patients in Lebanon were recovered and identified at the reference TB center of the Ministry of Public Health. All isolates were heat killed and subjected to DNA extraction. Spoligotyping method (TB-Spol, Beamedex, France) was used to identify the presence of 43 spacers via a multi-analyte profiling system (Luminex, Bio-Rad). Generated patterns were assigned to families using the SITVIT2 international database of the Pasteur Institute of Guadeloupe.

Results: The spoligotyping of the 50 MTB isolates revealed 13 lineages, one being novel. The most frequent shared-types (SIT) identified lineage was the Ural (34%), followed by the Central Asian lineage (10%) and a single isolate (2%) belonging to the rare Manu-Ancestor SIT523 lineage, associated with a highly virulent XDR MTB phenotype. The rest of the SIT isolates (18%) were equally distributed along 9 different lineages. The 13th non-SIT lineage is a novel one constituting 36% of the total isolates.

Conclusion:  The application of Spoligotyping Multiplex Luminex method is a novel, discriminatory and rapid method to use for genotyping of MTB isolates employing the multi-spacer analysis system. Our study showed genomic diversification of MTB isolates from Lebanon.

Author Biography

Ghassan M Matar, American University of Beirut
Professor Dept. of Microbiology and Immunology
Published
2018-02-22
How to Cite
1.
Massoud K, Araj GF, Fadlallah S, Reslan L, Itani L, Avedissian A, Dbaibo G, Saade A, Matar GM (2018) Genotyping of Mycobacterium tuberculosis in Lebanon using a novel rapid spoligotyping multiplex luminex method. J Infect Dev Ctries 12:18S. doi: 10.3855/jidc.10094
Section
The Lebanese LSIDCM