Increase of blaOXA-23-like in Acinetobacter baumannii at a tertiary care center in Lebanon between 2007 and 2013

  • Souha S Kanj Center for Infectious Diseases Research (CIDR), American University of Beirut Medical Center, Beirut, Lebanon
  • Ralph Tayyar Center for Infectious Diseases Research (CIDR), American University of Beirut Medical Center, Beirut, Lebanon
  • Marwa Shehab American University of Beirut
  • Bassam El-Hafi American University of Beirut http://orcid.org/0000-0003-3277-2948
  • Sari S Rasheed American University of Beirut
  • Kohar Annie Kissoyan American University of Beirut
  • Zeina A Kanafani American University of Beirut Medical Center
  • Rima Hanna Wakim American University of Beirut Medical Center
  • Nada Kara Zahreddine American University of Beirut Medical Center
  • George F Araj American University of Beirut Medical Center
  • Ghassan Dbaibo American University of Beirut Medical Center
  • Ghassan M Matar American University of Beirut
Keywords: a. baumannii, oxa-23, rapd

Abstract

Introduction: The multi-drug resistant nature of Acinetobacter baumannii isolates have rendered many broad-spectrum antimicrobial agents ineffective against them. The purpose of this retrospective study is to define and compare the molecular characteristics of A. baumannii isolates from patients at a tertiary care center in Lebanon from two outbreaks, the first in 2007-2008, as part of a case-controlled study involving A. baumannii cases admitted to the ICU, and the second in 2013.

Methodology: A total of 148 A. baumannii clinical isolates were collected from various clinical specimens during 2007-2008 and 2013. All A. baumannii isolates were screened for blaOXA-23-like and blaOXA-51-like genes of carbapenem resistance. Additionally, in an effort to assess the degree of the isolates’ genomic relatedness, random amplification of polymorphic DNA (RAPD) was performed.

Results: There was an increase in the prevalence of blaOXA-23-like and blaOXA-51-like genes between the two time periods; however, only 22% isolate genomic relatedness was calculated between 2007-2008 and 2013. Taking 80% as a margin of compatibility, 31 distinct clusters containing 2 to 11 strains were observed when both time periods were analyzed.

Conclusion: The presence of numerous clusters accompanied by a predominant increase in the prevalence of blaOXA-23-like between 2007 and 2013 suggests a horizontal transmission of the gene within various strains of the species, contributing to the persistent increase in carbapenem resistance over the years. Therefore, infection control measures are required with compliance among all healthcare workers.

Author Biography

Ghassan M Matar, American University of Beirut
Professor Dept. of Microbiology and Immunology
Published
2018-02-22
How to Cite
1.
Kanj SS, Tayyar R, Shehab M, El-Hafi B, Rasheed SS, Kissoyan KA, Kanafani ZA, Hanna Wakim R, Kara Zahreddine N, Araj GF, Dbaibo G, Matar GM (2018) Increase of blaOXA-23-like in Acinetobacter baumannii at a tertiary care center in Lebanon between 2007 and 2013. J Infect Dev Ctries 12:4S. doi: 10.3855/jidc.10100
Section
The Lebanese LSIDCM