Genotyping of Staphylococcus aureus associated with nasal colonization among healthcare workers using DNA microarray

  • Abiola Catherine Senok Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai, United Arab Emirates
  • Ali Somily King Khalid University Hospital and King Saud University, Riyadh, Saudi Arabia
  • Muhabat Raji Alfaisal University, Riyadh, Saudi Arabia
  • Ghada Garaween Alfaisal University, Riyadh, Saudi Arabia
  • Maha Kabil King Saud University Medical City, Riyadh Saudi Arabia
  • Atef Shibl Alfaisal University, Riyadh, Saudi Arabia
  • Stefan Monecke Abbott (Alere Technologies GmbH), Jena, Germany
  • Ralf Ehricht Abbott (Alere Technologies GmbH), Jena, Germany
Keywords: Staphylococcus aureus, MSSA, MRSA, clonal complex, DNA microarray, Saudi Arabia

Abstract

Introduction: Healthcare workers (HCWs) colonized with Staphylococcus aureus may serve as a reservoir of infection. This study was carried to determine the genetic make-up of S. aureus nasal colonizers in HCWs.

Methodology: Nasal swabs were obtained from 93 HCWs and molecular characterization of identified S. aureus isolates was carried out using the StaphyType DNA microarray (Alere Technologies GmbH, Jena, Germany).

Results: Twenty-nine HCWs (31%) were colonized with S. aureus (MSSA = 23; MRSA = 6). Thus the overall MRSA carriage rate was 6.5% (n/N = 6/93) and 20.7% (n/N = 6/29) of those colonized with S. aureus harboured MRSA. The S. aureus isolates belonged to 16 clonal complexes (CC). MSSA isolates included three each for CC15, CC188, ST2867; two each for CC5, CC97, CC367 as well as one each for CC1, CC8, CC30, CC45, CC101, CC121, ST291/813 and CC1153. The staphylococcal cassette chromosome recombinase genes ccrA-1; ccrB-1 and the fusidic acid resistance gene (fusC) were present in two MSSA isolates (CC1 and CC8). The six MRSA isolates included CC5-MRSA-[VI+fusC] (n = 2); one each of CC5-MRSA-V; CC22-MRSA-IV (tst1+); CC80-MRSA-IV [pvl+] (“European CA-MRSA Clone”) and CC97-MRSA-[V+fusC].

Conclusion: There is wide clonal diversity of S. aureus colonizers with associated high MRSA carriage among the HCWs. The presence of genetically stable MSSA isolates with the capability to transform into MRSA isolates is of concern.

Published
2018-05-31
How to Cite
1.
Senok A, Somily A, Raji M, Garaween G, Kabil M, Shibl A, Monecke S, Ehricht R (2018) Genotyping of Staphylococcus aureus associated with nasal colonization among healthcare workers using DNA microarray. J Infect Dev Ctries 12:321 - 325. doi: https://doi.org/10.3855/jidc.10328
Section
Original Articles