Occurrence of VIM-4 metallo-β-lactamase-producing Pseudomonas aeruginosa in an Algerian hospital

  • Manel Merradi Department of Microbiology and Biochemistry, Faculty of Natural and Life Sciences University of Batna 2, Batna, Algeria
  • Ahmed Kassah-Laouar Faculty of Medicine, University of Batna 2, Batna, Algeria
  • Ammar Ayachi Department of Veterinary Sciences,Veterinary and Agricultural Sciences Institute, University of Batna 1, Batna, Algeria
  • Nouzha Heleili Department of Veterinary Sciences,Veterinary and Agricultural Sciences Institute, University of Batna 1, Batna, Algeria
  • Taha Menasria Department of Applied Biology, Faculty of Natural and Life Sciences, University of Tebessa, Tebessa, Algeria
  • Didier Hocquet Hospital Hygiene, Regional University Hospital Center, Besançon, France
  • Pascal Cholley Hospital Hygiene, Regional University Hospital Center, Besançon, France
  • Marlène Sauget Hospital Hygiene, Regional University Hospital Center, Besançon, France
Keywords: Pseudomonas aeruginosa, antibiotic resistance, MBL, VIM-4, PFGE, sequence type

Abstract

Introduction: Pseudomonas aeruginosa is one of the most common nosocomial pathogens, known with a wide resistance to antimicrobials. Carbapenemases producing Pseudomonas aeruginosa is a growing global public health concern as this pathogen is easily transmissible among patients. Metallo-Beta-lactamases is the most important class of these carbapenemases with their broad-spectrum resistance profile. This study was conducted to investigate the prevalence of MBL-producing P. aeruginosa collected in an Algerian hospital.

Methodology: All Metallo-β-lactamase (MBL)-producing P. aeruginosa isolates recovered from patients during a 2 years period (2015-2016) were studied using a combination of phenotypic and molecular typing methods (susceptibility testing, molecular characterization of carbapenemase-encoding genes, multi-locus sequence typing and pulsed-field gel electrophoresis).

Results: A total of twenty-six MBL producing P. aeruginosa of 188 isolates were investigated. The burns unit ranked in the first position of the majority of identified cases with 73.07%. About 73.07% of total MBL isolates were mainly isolated from pus samples. The studied isolates were subjected to the molecular typing, in which 4 different Dra1-PFGE patterns and 3 sequences type were assigned (ST244, ST381, and ST1076), and all isolates were revealed positive for VIM-4.

Conclusions: We report the third description of blaVIM-4 in Algeria indicating the emergence and spread of carbapenemase-encoding genes among P. aeruginosa in the hospital environment.

Published
2019-04-30
How to Cite
1.
Merradi M, Kassah-Laouar A, Ayachi A, Heleili N, Menasria T, Hocquet D, Cholley P, Sauget M (2019) Occurrence of VIM-4 metallo-β-lactamase-producing Pseudomonas aeruginosa in an Algerian hospital. J Infect Dev Ctries 13:284-290. doi: 10.3855/jidc.10679
Section
Original Articles