Predominance of Central Asian strain (ST 26) in Mycobacterium tuberculosis isolates from Balochistan by spoligotyping

  • Muhammad Shafee Centre for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, Pakistan
  • Ferhat Abbas Centre for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, Pakistan
  • Zunera Tanveer Department of Physiology, Bloan University of Medical and Health Sciences, Quetta, Pakistan
  • Andrew Whitelaw Division of Medical Microbiology, University of Stellenbosch, Cape Town, South Africa
  • Lemese Ah Tow Division of Medical Microbiology, Department of Pathology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa
  • Muhammad Ashraf Fatima Jinnah Chest and General Hospital, Quetta, Pakistan
  • Irshad Ahmad School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, United Kingdom
  • Simon G Patching School of Biomedical Sciences and Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, United Kingdom https://orcid.org/0000-0001-6132-5317
  • Abdul Jabbar Department of Medical Lab Technology, University of Haripur, Haripur, Pakistan
  • Ali Akbar Department of Microbiology, Faculty of Life Sciences, University of Balochistan, Quetta, Pakistan
  • Mohammad Zahid Mustafa Centre for Advanced Studies in Vaccinology and Biotechnology (CASVAB), University of Balochistan, Quetta, Pakistan
Keywords: Genotyping, Spogliotyping, Mycobacterium tuberculosis, Central Asian Strain, Pakistan

Abstract

Introduction: Tuberculosis is a chronic debilitating infectious disease causing a severe challenge to public health, especially in developing countries. The aim of this study was to examine genetic diversity in Mycobacterium tuberculosis strains circulating in the Balochistan region of Pakistan.

Methodology: One hundred isolates collected from patients visiting the Fatima Jinnah TB Hospital in Quetta were subjected to genotype analysis by spoligotyping.

Results: Three main genotypes were identified: Central Asian Strain 1 (CAS1) (n = 89), East African Indian (EAI) strain (n = 7) and Latin American Mediterranean (LAM) strain (n = 3). The CAS1 clade (ST 26) had high genetic diversity represented by seven different spoligopatterns, of which one had major predominace (n = 75).

Conclusions: This is the first insight into the genotype of M. tuberculosis strains in the Balochistan region that might serve as a base line study for control of tuberculosis in the community.

Published
2019-07-31
How to Cite
1.
Shafee M, Abbas F, Tanveer Z, Whitelaw A, Tow LA, Ashraf M, Ahmad I, Patching SG, Jabbar A, Akbar A, Mustafa MZ (2019) Predominance of Central Asian strain (ST 26) in Mycobacterium tuberculosis isolates from Balochistan by spoligotyping. J Infect Dev Ctries 13:619-625. doi: 10.3855/jidc.10803
Section
Original Articles