In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium

  • Aysegul A Gokmen Department of Microbiology, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, Turkey
  • Hüseyin Can Molecular Biology Section, Department of Biology, Faculty of Science, Ege University, İzmir, Turkey
  • Hüsniye Kayalar Department of Pharmacognosy, Faculty of Pharmacy, Ege University, İzmir, Turkey
  • Bayram Pektaş Department of Microbiology, İzmir Atatürk Training and Research Hospital, Yeşilyurt, İzmir, Turkey
  • Selçuk Kaya Department of Microbiology, Faculty of Medicine, İzmir Katip Çelebi University, İzmir, Turkey
Keywords: Trichomanas vaginalis, Haplophyllum myrtifolium, antitrichomonal activity

Abstract

Introduction: In the classic treatment of Trichomonas vaginalis infection, although metronidazole has been used since the 1960s, there has been an increase in MTZ-resistant T. vaginalis strains and failure in the treatment of trichomoniasis causes serious concerns. Therefore, the present study aimed to investigate the in vitro antitrichomonal activities of extracts (ethanol and total alkaloid) and pure compounds (chrysosplenetin, dictamnine, gamma-Fagarine, skimmianine) of H. myrtifolium against T. vaginalis.

Methodology: H. myrtifolium was collected from the town of Honaz in Denizli, located in the Aegean region of Turkey, and preparation of extracts and isolation and structure elucidation of pure compounds were performed. Later, different concentrations of extracts and pure compounds were incubated with T. vaginalis trophozoites isolated from Turkey, which are known to be sensitive to metronidazole.

Results: It was found that ethanol extract caused a more effective lysis on T. vaginalis trophozoites compared with total alkaloid extract (P < 0.05). No compounds except for furoquinoline alkaloid skimmianine prepared above 37.5 µg/mL were found to have any inhibitory effect on T. vaginalis trophozoites.

Conclusion: The ethanol extract of H. myrtifolium and skimmianine can be considered as potential candidates for antitrichomonal drug development.

Published
2019-03-31
How to Cite
1.
Gokmen A, Can H, Kayalar H, Pektaş B, Kaya S (2019) In vitro anti-Trichomonas vaginalis activity of Haplophyllum myrtifolium. J Infect Dev Ctries 13:240-244. doi: https://doi.org/10.3855/jidc.10854
Section
Original Articles