Assessing the role of the RND efflux pump in metronidazole resistance of Helicobacter pylori by RT-PCR assay

  • Jalil Fallah Mehrabadi MARS bioinformatics Institute,Tehran
  • Mehrandokht Sirous School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Naser Ebrahimi Daryani Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
  • Saeed Eshraghi School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
  • Bahram Akbari School of Medicine, Tarbiat Modares University, Tehran, Iran
  • Mohammad Hasan Shirazi School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Keywords: H. pylori, Metronidazole, RND efflux pump

Abstract

Introduction: Metronidazole is a significant antibiotic used for eradication of Helicobactor pylori infections and it is of notice that metronidazole-resistant clinical isolates have been found in high rates worldwide. While the RND family of efflux pumps plays a central role in drug resistance among Gram-negative bacteria, this is questionable for H. pylori.

Methodology: To understand whether TolC homologues of RND pumps contribute to metronidazole resistance in H. pylori isolates, expression of four TolC homologous genes of five resistant clinical isolates exposed to varying concentrations of metronidazole were evaluated by RT-PCR and transcriptional analysis.

Results: The results indicate that excess amounts of metronidazole are able to increase the expression level of these genes at the transcriptional stage.

Conclusions: Therefore, it may be hypothesized that use of metronidazole in H. pyori infection can induce metronidazole resistance. Furthermore, the RND family of efflux pumps may contribute to metronidazole resistance in clinical isolates of H. pylori.

Published
2010-10-19
How to Cite
1.
Mehrabadi J, Sirous M, Daryani N, Eshraghi S, Akbari B, Shirazi M (2010) Assessing the role of the RND efflux pump in metronidazole resistance of Helicobacter pylori by RT-PCR assay. J Infect Dev Ctries 5:88-93. doi: https://doi.org/10.3855/jidc.1187
Section
Original Articles