Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina

Authors

  • Andrea Karina Merkier Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos Aires
  • Mariana Catalano Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos Aires
  • Maria Soledad Ramirez Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos Aires
  • Cecilia Quiroga Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos Aires
  • Betina Orman Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos Aires
  • Laura Ratier Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos Aires
  • Angela Famiglietti Laboratorio de Bacteriología, Departamento de Análisis Clínicos, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954. Buenos Aires
  • Carlos Vay Laboratorio de Bacteriología, Departamento de Análisis Clínicos, Hospital de Clínicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 954. Buenos Aires
  • Ana Di Martino Servicio de Infectología, Sanatorio Mitre, Bartolomé Mitre 365. Buenos Aires
  • Sara Kaufman Hospital Juan Fernández, Cerviño 3356. Buenos Aires
  • Daniela Centrón Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155. Buenos Aires

DOI:

https://doi.org/10.3855/jidc.269

Keywords:

Acinetobacter baumanni, carbapenemases, insertion sequence

Abstract

Background: In order to study the enzymatic carbapenem resistance mechanisms in Acinetobacter baumannii isolates from Argentina, we performed molecular characterization on 41 epidemiologically unrelated strains isolated from 1995 to 2006 with diminished susceptibilities to imipenem and meropenem. Methodology: Acinetobacter baumannii isolates were identified with the ARDRA technique. The total genomic DNA was used to detect each carbapenem β-lactamase gene described so far in this species and those insertion sequences usually associated to carbapenem β-lactamase genes (ISAba1, 2, 3, 4 and IS18) by the PCR technique with specific primers. Results: 26 out of 41 Acinetobacter baumannii isolates with diminished susceptibilities to carbapenems harboured the blaOXA-23 gene. The blaOXA-58 was detected in 13 out of 41 isolates. ISAba1 was always located upstream blaOXA-23. All isolates containing the blaOXA-58 gene showed ISAba3 downstream of the carbapenemase, while 4 isolates had a second copy of the ISAba3 upstream of the gene. Conclusion: Enzymatic carbapenem resistance in Acinetobacter baumannii was found in 88% of 41 non-epidemiologically-related strains mediated by the polyclonal spread of the blaOXA-23 and blaOXA-58 genes. The genetic structures surrounding the oxacillinase genes found in our bacterial isolates revealed a particular epidemiology in our geographical region. This data suggests the need of local molecular surveillance to help control multirresistance Acinetobacter baumannii infections.

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Published

2008-06-01

How to Cite

1.
Merkier AK, Catalano M, Ramirez MS, Quiroga C, Orman B, Ratier L, Famiglietti A, Vay C, Di Martino A, Kaufman S, Centrón D (2008) Polyclonal spread of blaOXA-23 and blaOXA-58 in Acinetobacter baumannii isolates from Argentina. J Infect Dev Ctries 2:235–240. doi: 10.3855/jidc.269

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Original Articles