Multilocus VNTR analysis of Mycobacterium ulcerans strains isolated in Côte d’Ivoire

Grossmann Marie-David Coulibaly-N´Golo; Euloge Ekaza; Bakary Coulibaly; N’guetta Aka; Raymond Kouassi N’Guessan; Jean Michel Thiberge; Valérie Caro; Sylvain Brisse; Mireille Bretin-Dosso

doi:10.3855/jidc.1364

Multilocus VNTR analysis of Mycobacterium ulcerans strains isolated in Côte d’Ivoire

Authors

Grossmann Marie-David Coulibaly-N´Golo Institut Pasteur de Côte d'Ivoire
Euloge Ekaza Institut Pasteur de Côte d'Ivoire
Bakary Coulibaly Institut Pasteur de Côte d'Ivoire
N’guetta Aka Institut Pasteur de Côte d'Ivoire
Raymond Kouassi N’Guessan Institut Pasteur de Côte d'Ivoire
Jean Michel Thiberge Institut Pasteur Paris
Valérie Caro Institut Pasteur Paris
Sylvain Brisse Institut Pasteur Paris
Mireille Bretin-Dosso Institut Pasteur de Côte d'Ivoire

DOI:

https://doi.org/10.3855/jidc.1364

Keywords:

Buruli ulcer, M. ulcerans, Cote d’Ivoire, MIRU VNTR typing, sequencing

Abstract

Introduction: Buruli ulcer, caused by Mycobacterium ulcerans, is endemic in more than 30 countries worldwide, with Côte d'Ivoire being among the most affected countries.

Methodology: We used seven variable number of tandem repeats (VNTR) markers and analyzed 114 samples from 11 Ivorian localities consisting of 33 bacterial strains and 81 clinical samples. Complete data sets at loci 1, 6, 9 and 33 were obtained for 18 of these strains (n = 15) and samples (n = 3) collected in each of the localities.

Results: All the strains had allelic profile [3113], corresponding to the previously described Atlantic Africa genotype.

Conclusion: Sequencing of PCR products at all loci showed no variation in sequence or repeat number, underlining the genetic monomorphism of M. ulcerans in Côte d'Ivoire.

Author Biographies

Grossmann Marie-David Coulibaly-N´Golo, Institut Pasteur de Côte d'Ivoire

Bacteriology and Virology Department

Euloge Ekaza, Institut Pasteur de Côte d'Ivoire

Bacteriology and Virology Department

Bakary Coulibaly, Institut Pasteur de Côte d'Ivoire

Bacteriology and Virology Department

N’guetta Aka, Institut Pasteur de Côte d'Ivoire

Bacteriology and Virology Department

Raymond Kouassi N’Guessan, Institut Pasteur de Côte d'Ivoire

Bacteriology and Virology Department

Jean Michel Thiberge, Institut Pasteur Paris

Genotyping of Pathogens and Public Health

Valérie Caro, Institut Pasteur Paris

Genotyping of Pathogens and Public Health

Sylvain Brisse, Institut Pasteur Paris

Genotyping of Pathogens and Public Health

Mireille Bretin-Dosso, Institut Pasteur de Côte d'Ivoire

Bacteriology and Virology Department

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Published

2010-10-07

How to Cite

Coulibaly-N´Golo GM-D, Ekaza E, Coulibaly B, Aka N, N’Guessan RK, Thiberge JM, Caro V, Brisse S, Bretin-Dosso M (2010) Multilocus VNTR analysis of Mycobacterium ulcerans strains isolated in Côte d’Ivoire. J Infect Dev Ctries 5:059–063. doi: 10.3855/jidc.1364

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Issue

Vol. 5 No. 01: January 2011

Section

Brief Original Articles

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