Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil

Rodrigo Prado Martins; Maria Cristina da Silva; Valeria Dutra; Luciano Nakazato; Domingos da Silva Leite

doi:10.3855/jidc.1217

Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil

Authors

Rodrigo Prado Martins Universidade Federal de Mato Grosso, Brazil
Maria Cristina da Silva Universidade Federal de Mato Grosso, Brazil
Valeria Dutra Universidade Federal de Mato Grosso, Brazil
Luciano Nakazato Universidade Federal de Mato Grosso, Brazil
Domingos da Silva Leite Instituto de Biologia, Universidade Estadual de Campinas, Brazil

DOI:

https://doi.org/10.3855/jidc.1217

Keywords:

STEC, ETEC, PCR, slaughterhouse, Mato Grosso.

Abstract

Introduction: This study aimed to estimate the prevalence of enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing Escherichia coli (STEC) strains in pigs slaughtered in abattoirs located in the state of Mato Grosso, Brazil.

Methodology: Intestinal samples from 74 animals were aseptically dissected and lumen content was plated on MacConkey agar. Confluent colonies from each plate were screened for the presence of ETEC and STEC strains by PCR assays.

Results: It was verified that the prevalence of STEC and ETEC carriers was 1.35% and 9.46% respectively. One (1.35%) of the 74 samples tested was positive for the stx2 gene, and seven (9.46%) for st1, of which two (2.70%) were also positive for lt1.

Conclusion: The results provided represent a benchmark for future research on pathogenic E. coli of porcine origin in Mato Grosso.

Downloads

Published

2010-07-19

How to Cite

Martins RP, da Silva MC, Dutra V, Nakazato L, Leite D da S (2010) Prevalence of enterotoxigenic and Shiga toxin-producing Escherichia coli in pigs slaughtered in Mato Grosso, Brazil. J Infect Dev Ctries 5:123–127. doi: 10.3855/jidc.1217

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Vol. 5 No. 02: February 2011

Section

Brief Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).