Molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in Panama: May – June 2008

  • Alexander A Martinez Gorgas Memorial Institute for Health Studies, Panama City, Panama
  • Juan Castillo Gorgas Memorial Institute for Health Studies, Panama City, Panama
  • Mirla C Sanchez Gorgas Memorial Institute for Health Studies, Panama City, Panama
  • Yamitzel Zaldivar Gorgas Memorial Institute for Health Studies, Panama City, Panama
  • Yaxelis Mendoza Gorgas Memorial Institute for Health Studies, Panama City, Panama
  • Maribel Tribaldos Gorgas Memorial Institute for Health Studies, Panama City, Panama
  • Pablo Acosta Ministry of Health, Chiriqui, Panama
  • Rebecca E Smith Gorgas Memorial Institute for Health Studies, Panama City, Panama
  • Juan Miguel Pascale Gorgas Memorial Institute for Health Studies, Panama City, Panama
Keywords: meningitis, aseptic, enterovirus B, human, echovirus infections, Panama, disease outbreaks, VP1

Abstract

Introduction: Aseptic meningitis outbreaks are commonly caused by viral pathogens with enterovirus a common etiological agent. Between May and June of 2008, an outbreak of 173 cases of aseptic meningitis occurred in the Chiriqui Province of Panama. Molecular techniques were used to identify the etiological agent.

Methodology: Cerebrospinal fluid (CSF) samples from 75 patients were received at the Gorgas Memorial Institute for Health Studies.  RNA extraction and one-step RT-PCR were performed on each sample to determine the presence of enterovirus.  Thirty-four samples which were positive for enterovirus were subject to group-specific PCR, sequencing, and phylogenetic analysis to identify the etiological agent of the outbreak.

Results: The CSF of 58 subjects was found positive for the enterovirus family using RT-PCR. Thirty-four samples were found to belong to the enterovirus B group. Phylogenetic analysis of four successfully sequenced samples revealed echovirus 30 as the etiological agent. Conclusion: Echovirus 30 is reported as the likely cause of an outbreak of aseptic meningitis in Panama, the first since the 1980s.

Author Biography

Juan Miguel Pascale, Gorgas Memorial Institute for Health Studies, Panama City, Panama
Published
2012-12-15
How to Cite
1.
Martinez AA, Castillo J, Sanchez MC, Zaldivar Y, Mendoza Y, Tribaldos M, Acosta P, Smith RE, Pascale JM (2012) Molecular diagnosis of echovirus 30 as the etiological agent in an outbreak of aseptic meningitis in Panama: May – June 2008. J Infect Dev Ctries 6:836-841. doi: 10.3855/jidc.2615
Section
Outbreak