Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6

  • Atef M El-Gendy US Naval Medical Research Unit No. 3, Cairo, Egypt
  • Adel Mansour US Naval Medical Research Unit No. 3, Cairo, Egypt
  • Hind I Shaheen US Naval Medical Research Unit No. 3, Cairo, Egypt
  • Marshall R Monteville US Naval Medical Research Unit No. 3, Cairo, Egypt
  • Adam W Armstrong US Naval Medical Research Unit No. 3, Cairo, Egypt
  • Nasr El-Sayed Egyptian Ministry of Health, Cairo, Egypt.
  • Sylvia Y. N Young US Naval Medical Research Unit No. 3, Cairo, Egypt
  • John D Klena US Naval Medical Research Unit No. 3, Cairo, Egypt

Abstract

Introduction: One approach to control enterotoxigenic Escherichia coli (ETEC) infections has been to develop vaccines focused on inducing protective immunity against surface expressed antigenic factors. One such factor is coli surface antigen 6 (CS6); ETEC isolates expressing CS6 may also simultaneously co-express surface antigens CS4 or CS5. However, there is little information regarding the inter-relationships of isolates expressing the CS6 antigen alone or in combination with CS4 or CS5.

Methodology: A total of 62 CS6-associated ETEC isolates were evaluated for their antimicrobial susceptibility, mechanisms of resistance, toxin genes, colonization factor expression, and XbaI-pulsed-field gel electrophoretic profiles.

Results: We observed 46 XbaI profiles; 31 were exclusive to ETEC expressing CS6 alone and 15 among the ETEC co-expressing CS4 or CS5. Nearly half (47%) of these isolates were resistant to ampicillin, a third (37%) of the isolates were resistant to trimethoprim-sulfamethoxazole, and 24% of the isolates were tetracycline-resistant. A blaTEM gene was detected in 24 (83%) ampicillin-resistant isolates. Trimethoprim-sulfamethoxazole-resistant isolates (n = 23) carried either sulI (n = 1, 4%), sulII (n = 8, 35%) or both genes (n = 10, 43%); 4 had no detectable sul gene.

Conclusions: Our results show a lack of clonality among Egypt CS6 E. coli isolates and supports the use and the further research on vaccines targeting this cell surface antigen.

Author Biographies

Atef M El-Gendy, US Naval Medical Research Unit No. 3, Cairo, Egypt
Head, Bacteriology Section.Bacterial and Parasitic Diseases Program.United States Naval Medical Research Unit No. 3, Cairo, Egypt; (NAMRU-3).
Adel Mansour, US Naval Medical Research Unit No. 3, Cairo, Egypt

Adel Mansour, Clinical Trials and Military Studies Program, US Naval Medical Research Unit No. 3, Cairo, Egypt.

Hind I Shaheen, US Naval Medical Research Unit No. 3, Cairo, Egypt

Clinical Trials and Military Studies Program, US Naval Medical Research Unit No. 3, Cairo, Egypt.

Marshall R Monteville, US Naval Medical Research Unit No. 3, Cairo, Egypt

Clinical Trials and Military Studies Program, US Naval Medical Research Unit No. 3, Cairo, Egypt. Current address: US Navy Environmental and Preventive Medicine Unit 6, Honolulu, HI, USA.

Adam W Armstrong, US Naval Medical Research Unit No. 3, Cairo, Egypt

Clinical Trials and Military Studies Program, US Naval Medical Research Unit No. 3, Cairo, Egypt. 4 Current address: National Naval Medical Center, US Navy Central HIV Program, Bethesda, MD, USA.

Nasr El-Sayed, Egyptian Ministry of Health, Cairo, Egypt.

Nasr El-Sayed , Egyptian Ministry of Health, Cairo, Egypt.

Sylvia Y. N Young, US Naval Medical Research Unit No. 3, Cairo, Egypt

Sylvia Y. N. Young,Clinical Trials and Military Studies Program, US Naval Medical Research Unit No. 3, Cairo, Egypt. Current address: US Navy Environmental and Preventive Medicine Unit 6, Honolulu, HI, USA.

John D Klena, US Naval Medical Research Unit No. 3, Cairo, Egypt

Clinical Trials and Military Studies Program, US Naval Medical Research Unit No. 3, Cairo, Egypt. Current address: Unit 7300 Box 060 DPO AP 96521-0060 USA; +86 139 1101 2902 (telephone); +8610 8532 5363 (fax)

Published
2013-02-15
How to Cite
El-Gendy A, Mansour A, Shaheen H, Monteville M, Armstrong A, El-Sayed N, Young S, Klena J (2013) Genotypic characterization of Egypt enterotoxigenic Escherichia coli isolates expressing coli surface antigen 6. The Journal Of Infection In Developing Countries 7 (02): 090-100. https://doi.org/10.3855/jidc.2454
Section
Original Articles

Keywords

Enterotoxigenic Escherichia coli; children; diarrhea; Egypt