Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan

Adnan Khan; Amber Farooqui; Yasir Raza; Faisal Rasheed; Hamid Manzoor; Syed Shakeel Akhtar; Muhammad Saeed Quraishy; Salvatore Rubino; Shahana U Kazmi; Bianca Paglietti

doi:10.3855/jidc.2942

Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan

Authors

Adnan Khan Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Pakistan
Amber Farooqui Division of Immunology, International Institute of Infection and Immunity, Shantou University Medical College, Xinling Road 22, Shantou, Guangdong 515041
Yasir Raza Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi
Faisal Rasheed Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi
Hamid Manzoor Department of Surgery, Civil Hospital, Karachi
Syed Shakeel Akhtar Department of Surgery, Civil Hospital, Karachi
Muhammad Saeed Quraishy Department of Surgery, Civil Hospital, Karachi
Salvatore Rubino Department of Biomedical Sciences, University of Sassari, Sassari, Italy
Shahana U Kazmi Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi
Bianca Paglietti Department of Biomedical Sciences, University of Sassari, Sassari,

DOI:

https://doi.org/10.3855/jidc.2942

Keywords:

H. pylori, cagA, vacA, duodenal ulcer, gastric ulcer, Pakistan

Abstract

Introduction: The etiological association of Helicobacter pylori with gastric ulcer (GU), gastric cancer (GC), and duodenal ulcer (DU) is well-known. Understanding the epidemiology of H. pylori facilitates the estimation of disease burden in a certain population. This study presents the diversity of H. pylori genotypes and their association with different clinical outcomes among dyspeptic patients in Pakistan over a period of four years.

Methodology: Gastric biopsy samples from a total of 450 dyspeptic individualswere subjected to PCR, genotypingand histology.

Results: A total of 201 (45%) cases were found positive for H. pylori. The detection rate was high in GU (91%), DU (86%) and GC (83%) cases compared with those cases who had intact gastric mucosa (18%). Histology revealed the presence of infection in 68% of cases of mild/chronic nonspecific gastritis with others belonging to the GU sequel. cagA gene carriage was observed in 104 (51%) cases or mostly from DU, GU and GC groups, of which 97 were Western type strains while 3 were East-Asian type strains that are rarely observed in South Asia. vacA allelic variant s1am1 was most commonly observed, followed by s1am2, and s1bm1, with direct correlation in diseased cases (gastritis, GU, DU and GC). Prevalent genotypic combinations were s1am1/cagA^- in gastritis and s1am1/cagA⁺ in DU, GU, and GC.

Conclusions: Our study indicates the predominant circulation of Western type cagA and vacAs1am1 type H. pylori strains in Pakistan.

Author Biographies

Adnan Khan, Immunology and Infectious Diseases Research Laboratory, Department of Microbiology, University of Karachi, Pakistan

Lecturer

Department of Microbiology

Muhammad Saeed Quraishy, Department of Surgery, Civil Hospital, Karachi

Downloads

Published

2013-03-14

How to Cite

Khan A, Farooqui A, Raza Y, Rasheed F, Manzoor H, Akhtar SS, Quraishy MS, Rubino S, Kazmi SU, Paglietti B (2013) Prevalence, diversity and disease association of Helicobacter pylori in dyspeptic patients from Pakistan. J Infect Dev Ctries 7:220–228. doi: 10.3855/jidc.2942

Download Citation

Endnote/Zotero/Mendeley (RIS)

Issue

Vol. 7 No. 03: March 2013

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).