Investigation of a food-borne Salmonella Oranienburg outbreak in a Mexican prison

  • Ma. Soledad Vázquez-Garcidueñas Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, Mexico
  • Nallely Lizbeth Romero-Pérez Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, Mexico
  • Gloria Alicia Figueroa-Aguilar Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, Mexico
  • Juan Luis Jaime-Sánchez Laboratorio Estatal de Salud Pública, Secretaría de Salud del Estado de Michoacán, Mexico
  • Gerardo Vázquez-Marrufo Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, Mexico
Keywords: Salmonella serotype Oranienburg, prison outbreak, ERIC-BOX-RAPD, multiple antibiotic resistance, Mexico

Abstract

Introduction: Gastroenteritis outbreaks in prisons represent a public health risk worldwide. Identifying and characterizing the etiological agents of gastroenteritis outbreaks in prisons is important for implementing effective prevention and infection control measures. We present the first studied case of a gastroenteritis outbreak in a Mexican prison.

Methodology: Rectal swab samples were obtained from affected inmates. Standard microbiological techniques were used for isolating Salmonella enterica. Isolates were typed by PCR assays of DNA repetitive elements (ERIC, BOX, REP) and RAPD. Antibiotic resistance profiles were performed by the Kirby-Bauer method.

Results: S. enterica serotype Oranienburg was responsible for the outbreak affecting 150 inmates. All patients presented diarrhea, and 70% of them also presented vomiting, with no fatal cases. The origin of the outbreak was undetermined due to the difficulty of gathering epidemiological information, but was likely the result of consumption of shrimp broth or a cantaloupe melon beverage. REP, BOX, and ERIC analyses of 26 serotype Oranienburg strains resulted in Simpson discrimination index (D) values of 0, 0.5507, and 0.5661, respectively. The D values from DG93-RAPD analyses and from the combined ERIC-BOX-DG93 markers were 0.7753 and 0.6092, respectively. All strains showed multiresistance to antibiotics.

Conclusions: This is the only studied case of a gastroenteritis outbreak in a Mexican prison, and of the first such outbreak caused by serotype Oranienburg. The combined ERIC, BOX, and RAPD markers adequately assessed the genotype diversity of analyzed strains. Penitentiary personnel or inmates involved in outbreaks might spread multiresistant strains outside of the facility.

Author Biographies

Ma. Soledad Vázquez-Garcidueñas, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, Mexico

División de Estudios de Posgrado, Facultad de Ciencias Médicas y Biológicas “Dr. Ignacio Chávez”

Researcher

Nallely Lizbeth Romero-Pérez, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, Mexico

Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia

Graduate student

Gloria Alicia Figueroa-Aguilar, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, Mexico

Laboratorio Estatal de Salud Pública

Head of the Laboratory

Juan Luis Jaime-Sánchez, Laboratorio Estatal de Salud Pública, Secretaría de Salud del Estado de Michoacán, Mexico

Laboratorio Estatal de Salud Pública

Laboratory technician

Gerardo Vázquez-Marrufo, Universidad Michoacana de San Nicolás de Hidalgo, Michoacán, Mexico

Centro Multidisciplinario de Estudios en Biotecnología, Facultad de Medicina Veterinaria y Zootecnia

Researcher

Published
2014-02-13
How to Cite
1.
Vázquez-Garcidueñas MS, Romero-Pérez NL, Figueroa-Aguilar GA, Jaime-Sánchez JL, Vázquez-Marrufo G (2014) Investigation of a food-borne Salmonella Oranienburg outbreak in a Mexican prison. J Infect Dev Ctries 8:143-153. doi: 10.3855/jidc.3367
Section
Original Articles