Emergence and clonal dissemination of Salmonella enterica serovar Enteritidis causing salmonellosis in Mauritius

  • Mohammad I Issack Central Health Laboratory, Victoria Hospital, Candos, Mauritius
  • Rene S Hendriksen National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark
  • Eija Hyytiä-Trees Centers for Disease Control and Prevention, Enteric Diseases Laboratory Branch, Atlanta, Georgia, United States
  • Christina A Svendsen National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark
  • Matthew Mikoleit Centers for Disease Control and Prevention, Enteric Diseases Laboratory Branch, Atlanta, Georgia, United States
Keywords: Salmonella Enteritidis, antimicrobial resistance, human infections, MLVA, Mauritius

Abstract

Introduction: For decades, Salmonella enterica serovar Enteritidis has been among the most prevalent serovars reported worldwide. However, it was rarely encountered in Mauritius until 2007; since then the number of non-typhoidal Salmonella serogroup O:9 (including serovar Enteritidis) increased. A study was conducted to investigate the genetic relatedness between S. Enteritidis isolates recovered in Mauritius from food and clinical specimens (stool, blood, and exudate).

Methodology: Forty-seven isolates of S. Enteritidis obtained in 2009 from human stools, blood cultures and exudates, and from food specimens were characterized by antimicrobial susceptibility testing and Multiple-Locus Variable-number tandem repeat Analysis (MLVA).

Results: With the exception of a single isolate which demonstrated intermediate susceptibility to streptomycin, all isolates were pansusceptible to the 14 antimicrobials tested. Thirty seven out of the 47 isolates (78.7%) exhibited an indistinguishable MLVA profile which included isolates from ready-to-eat food products, chicken, and human clinical isolates from stool, blood and exudate.

Conclusions: The presence of highly related strains in both humans and raw chicken, and the failure to isolate the serovar from other foods, suggests that poultry is the main reservoir of S. Enteritidis in Mauritius and that the majority of human cases are associated with chicken consumption which originated from one major producer. Stool isolates were indistinguishable or closely related to blood and exudate isolates, indicating that, besides gastroenteritis, the same strain caused invasive infections. Control of S.Enteritidis by poultry breeders would lower the financial burden associated with morbidity in humans caused by this organism in Mauritius.

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Author Biographies

Mohammad I Issack, Central Health Laboratory, Victoria Hospital, Candos, Mauritius

Consultant Pathologist

Head of Bacteriology Section

Rene S Hendriksen, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark
WHO Collaborating Centre for antimicrobial resistance in foodborne pathogens and European Union Reference Laboratory for antimicrobial resistance,
Eija Hyytiä-Trees, Centers for Disease Control and Prevention, Enteric Diseases Laboratory Branch, Atlanta, Georgia, United States
National Center for Emerging and Zoonotic Infectious Diseases; Division of Foodborne, Waterborne, and Environmental Diseases; Enteric Diseases Laboratory Branch.
Christina A Svendsen, National Food Institute, Technical University of Denmark, Kgs. Lyngby, Denmark

WHO Collaborating Centre for antimicrobial resistance in foodborne pathogens and European Union Reference Laboratory for antimicrobial resistance

Matthew Mikoleit, Centers for Disease Control and Prevention, Enteric Diseases Laboratory Branch, Atlanta, Georgia, United States
National Center for Emerging and Zoonotic Infectious Diseases; Division of Foodborne, Waterborne, and Environmental Diseases; Enteric Diseases Laboratory Branch.  Atlanta, Georgia
Published
2014-04-15
How to Cite
1.
Issack MI, Hendriksen RS, Hyytiä-TreesE, Svendsen CA, Mikoleit M (2014) Emergence and clonal dissemination of Salmonella enterica serovar Enteritidis causing salmonellosis in Mauritius. J Infect Dev Ctries 8:454-460. doi: 10.3855/jidc.3695
Section
Original Articles