Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia

  • Tengku Ahmad Akram Tengku Mohd Ariffin Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Suharni Mohamad Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Wan Nazirah Wan Yusuf Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
  • Rafidah Hanim Shueb Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia
Keywords: HIV-1, CRF33_01B, drug resistance, prisoners

Abstract

Introduction: The widespread use of highly active antiretroviral therapy (HAART) and continuous reports of HIV-1 strains developing resistance to these drugs is rather alarming, as transmission of resistant viruses to newly infected persons is possible. This study aimed to determine HIV-1 subtypes and the prevalence of primary mutations associated with antiretroviral (ARV) resistance among treatment-naive prisoners on the east coast of Malaysia.

Methodology: Viral RNA was extracted from plasma samples of 21 treatment-naive prisoners. Protease (PR) and reverse transcriptase (RT) regions were amplified and sequenced. Stanford HIV database algorithms were used for interpretation of resistance, and phylogenetic analysis was performed for subtype assignment.

Results: In the PR gene, no antiviral resistance-associated mutation was detected. For RT-associated mutations, K103N was the most prevalent in sequenced samples (14.3%). Genetic subtyping on the pol gene revealed that the majority of the prisoners were infected with subtype CRF33_01B (52.4%).

Conclusion: Continuous surveillance of newly infected individuals is required to help strategize the best antiviral treatment for these patients.

Author Biographies

Tengku Ahmad Akram Tengku Mohd Ariffin, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia

Master degree candidate in Medical Microbiology

Department of Medical Microbiology and Parasitology

Suharni Mohamad, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia

Lecturer in Parasitology

School of Dental Sciences

Wan Nazirah Wan Yusuf, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia

Lecturer in Pharmacology

Department of Pharmacology

Rafidah Hanim Shueb, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia

Lecturer in Clinical Virology

Department of Medical Microbiology and Parasitology

Published
2014-08-13
How to Cite
1.
Ariffin TAATM, Mohamad S, Yusuf WNW, Shueb RH (2014) Antiretroviral drug resistance and HIV-1 subtypes among treatment-naive prisoners in Kelantan, Malaysia. J Infect Dev Ctries 8:1063-1067. doi: 10.3855/jidc.4095
Section
Brief Original Articles