Phenotypic and genotypic analysis of bio-serotypes of Yersinia enterocolitica from various sources in Brazil

  • Leonardo Alves Rusak Fiocruz – Instituto Oswaldo Cruz, Laboratório de Pesquisa em Infecção Hospitalar – LAPIH, Rio de Janeiro, Brasil
  • Cristhiane Moura Falavina dos Reis Fiocruz – Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria – LABZOO, Rio de Janeiro, Brasil
  • André Victor Barbosa Universidade Federal Fluminense, Centro de Ciências Médicas, Instituto Biomédico, Niterói, Brasil
  • André Felipe Mercês Santos Fiocruz – Instituto Oswaldo Cruz, Laboratório de Referência Nacional em Cólera e outras enterobactérias, Rio de Janeiro, Brasil
  • Renata Paixão Faculdades Metropolitanas Unidas (FMU), Faculdade de Medicina Veterinária, São Paulo, Brasil
  • Ernesto Hofer Fiocruz – Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria – LABZOO, Rio de Janeiro, Brasil
  • Deyse Christina Vallim Fiocruz – Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria – LABZOO, Rio de Janeiro, Brasil
  • Marise Dutra Asensi Fiocruz – Instituto Oswaldo Cruz, Laboratório de Pesquisa em Infecção Hospitalar – LAPIH, Rio de Janeiro, Brasil
Keywords: Yersinia, zoonosis, antibiotics, PCR, PFGE

Abstract

Introduction: Yersinia enterocolitica is a well-known foodborne pathogen widely distributed in nature with high public health relevance, especially in Europe.

Methodology: This study aimed to analyze the pathogenic potential of Y. enterocolitica isolated strains from human, animal, food, and environmental sources and from different regions of Brazil by detecting virulence genes inv, ail, ystA, and virF through polymerase chain reaction (PCR), phenotypic tests, and antimicrobial susceptibility analysis. Pulsed-field gel electrophoresis (PFGE) was used for the assessment of phylogenetic diversity.

Results: All virulence genes were detected in 11/60 (18%) strains of serotype O:3, biotype 4 isolated from human and animal sources. Ten human strains (4/O:3) presented three chromosomal virulence genes, and nine strains of biotype 1A presented the inv gene. Six (10%) strains were resistant to sulfamethoxazole-trimethoprim, seven (12%) to tetracycline, and one (2%) to amikacin, all of which are used to treat yersiniosis. AMP-CEF-SXT was the predominant resistance profile. PFGE analysis revealed 36 unique pulsotypes, grouped into nine clusters (A to I) with similarity ≥ 85%, generating a diversity discriminatory index of 0.957. Cluster A comprised all bio-serotype 4/O:3 strains isolated from animal and humans sources.

Conclusions: This study shows the existence of strains with the same genotypic profiles, bearing all virulence genes, from human and animal sources, circulating among several Brazilian states. This supports the hypothesis that swine is likely to serve as a main element in Y. enterocolitica transmission to humans in Brazil, and it could become a potential threat to public health as in Europe.

Author Biographies

Leonardo Alves Rusak, Fiocruz – Instituto Oswaldo Cruz, Laboratório de Pesquisa em Infecção Hospitalar – LAPIH, Rio de Janeiro, Brasil
Laboratório de Pesquisa em Infecção Hospitalar – LAPIH/ Laboratório de Zoonoses Bacterianas/Setor Listeria - LABZOO
Cristhiane Moura Falavina dos Reis, Fiocruz – Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria – LABZOO, Rio de Janeiro, Brasil
Laboratório de Zoonoses Bacterianas/Setor Listeria - LABZOO.
André Victor Barbosa, Universidade Federal Fluminense, Centro de Ciências Médicas, Instituto Biomédico, Niterói, Brasil
Centro de Ciências Médicas, Instituto Biomédico.
André Felipe Mercês Santos, Fiocruz – Instituto Oswaldo Cruz, Laboratório de Referência Nacional em Cólera e outras enterobactérias, Rio de Janeiro, Brasil
Laboratório de Referência Nacional em Cólera e outras enterobactérias. Rio de Janeiro.
Renata Paixão, Faculdades Metropolitanas Unidas (FMU), Faculdade de Medicina Veterinária, São Paulo, Brasil
Faculdade de Medicina Veterinária.
Ernesto Hofer, Fiocruz – Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria – LABZOO, Rio de Janeiro, Brasil
Laboratório de Zoonoses Bacterianas/Setor Listeria - LABZOO.
Deyse Christina Vallim, Fiocruz – Instituto Oswaldo Cruz, Laboratório de Zoonoses Bacterianas/Setor Listeria – LABZOO, Rio de Janeiro, Brasil
Laboratório de Zoonoses Bacterianas/Setor Listeria - LABZOO.
Marise Dutra Asensi, Fiocruz – Instituto Oswaldo Cruz, Laboratório de Pesquisa em Infecção Hospitalar – LAPIH, Rio de Janeiro, Brasil
Laboratório de Pesquisa em Infecção Hospitalar – LAPIH
Published
2014-12-15
How to Cite
1.
Rusak LA, dos Reis CMF, Barbosa AV, Santos AFM, Paixão R, Hofer E, Vallim DC, Asensi MD (2014) Phenotypic and genotypic analysis of bio-serotypes of Yersinia enterocolitica from various sources in Brazil. J Infect Dev Ctries 8:1533-1540. doi: 10.3855/jidc.4553
Section
Original Articles