Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa

  • Anthony Marius Smith Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service, Johannesburg, South Africa
  • Husna Ismail Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service, Johannesburg, South Africa
  • Maryke M Henton Idexx Laboratories, Johannesburg, South Africa
  • Karen H Keddy Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service, Johannesburg, South Africa
  • GERMS-SA Surveillance Network
Keywords: Salmonella Enteritidis, wild animal, pulsed-field gel electrophoresis, PFGE, PulseNet

Abstract

Introduction: Salmonella is well recognized as an aetiological agent of gastrointestinal and diarrhoeal disease. Salmonella enterica serotype Enteritidis (Salmonella Enteritidis) is one of the commonest serotypes associated with foodborne illness. In South Africa, we compared Salmonella Enteritidis strains isolated from humans with gastroenteritis and strains isolated from captive wild animals, between June 2011 and July 2012.

Methodology: Bacteria were phenotypically characterized using standard microbiological techniques. Genotypic relatedness of isolates was investigated by pulsed-field gel electrophoresis (PFGE) analysis.

Results: a diversity of 27 PFGE patterns amongst 196 human non-invasive isolates was shown; two PFGE patterns predominated and accounted for 74% of all human isolates. Human isolates showed a 12% prevalence rate for nalidixic acid resistance. Animal isolates from 5 different sources were investigated. With the exception of an isolate from a ground hornbill, all animal isolates (jaguar, crocodile, lion and poultry) showed PFGE pattern matches to a human isolate. Animal isolates showed susceptibility to all antimicrobial agents tested, with the exception of nalidixic acid resistance in isolates from the lion and poultry source.

Conclusions: Our data showed similarities between Salmonella Enteritidis strains isolated from humans and captive wild animals, suggesting a probable common source for strains from humans and animals.

Author Biography

Anthony Marius Smith, Centre for Enteric Diseases, National Institute for Communicable Diseases, Division in the National Health Laboratory Service, Johannesburg, South Africa
Centre for Enteric Diseases; senior scientist
Published
2014-12-15
How to Cite
1.
Smith AM, Ismail H, Henton MM, Keddy KH, Surveillance Network G-S (2014) Similarities between Salmonella Enteritidis isolated from humans and captive wild animals in South Africa. J Infect Dev Ctries 8:1615-1619. doi: 10.3855/jidc.5393
Section
Brief Original Articles