Detection of plasmid-mediated quinolone resistance determinants and qnrS expression in Enterobacteriaceae clinical isolates

  • Xiaobing Jiang College of Life Sciences, Henan Normal University, Xinxiang, China
  • Jun Li Medical Administrative Department of Navy General Hospital, Beijing, China
  • Yunshan Zhang Central Laboratory of Navy General Hospital, Beijing, China
  • He Yan College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China
  • Yuxiao Wang Central Laboratory of Navy General Hospital, Beijing, China
  • Lei Shi College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China
  • Lijun Zhou Central Laboratory of Navy General Hospital, Beijing, China
Keywords: Enterobacteriaceae, PMQR, qnrS, expression

Abstract

Introduction: Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates.

Methodology: A total of 149 clinical isolates of Enterobacteriaceae were collected in Beijing and screened for PMQR genes usingpolymerase chain reaction (PCR). Real-time quantitative PCR was used to study the expression of qnrS.

Results: The rates of qnr and aac(6’)-Ib-cr genes were 7.4% and 8.1%, respectively. The higher basal expression of qnrS was observed in transconjugant strains, which had higher minimum inhibitory concentrations (MICs) of quinolones. Furthermore, qnrS expression levels increased in all three isolates when a quinolone was present.

Conclusions: Our data suggest that the level of qnrS expression was associated with quinolone resistance.

Published
2014-12-15
How to Cite
1.
Jiang X, Li J, Zhang Y, Yan H, Wang Y, Shi L, Zhou L (2014) Detection of plasmid-mediated quinolone resistance determinants and qnrS expression in Enterobacteriaceae clinical isolates. J Infect Dev Ctries 8:1625-1629. doi: 10.3855/jidc.4617
Section
Brief Original Articles