Detection of plasmid-mediated quinolone resistance determinants and qnrS expression in Enterobacteriaceae clinical isolates

Xiaobing Jiang; Jun Li; Yunshan Zhang; He Yan; Yuxiao Wang; Lei Shi; Lijun Zhou

doi:10.3855/jidc.4617

Detection of plasmid-mediated quinolone resistance determinants and qnrS expression in Enterobacteriaceae clinical isolates

Authors

Xiaobing Jiang College of Life Sciences, Henan Normal University, Xinxiang, China
Jun Li Medical Administrative Department of Navy General Hospital, Beijing, China
Yunshan Zhang Central Laboratory of Navy General Hospital, Beijing, China
He Yan College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China
Yuxiao Wang Central Laboratory of Navy General Hospital, Beijing, China
Lei Shi College of Light Industry and Food Sciences, South China University of Technology, Guangzhou, China
Lijun Zhou Central Laboratory of Navy General Hospital, Beijing, China

DOI:

https://doi.org/10.3855/jidc.4617

Keywords:

Enterobacteriaceae, PMQR, qnrS, expression

Abstract

Introduction: Rapid dissemination of plasmid-mediated quinolone resistance (PMQR) has been reported in clinical isolates.

Methodology: A total of 149 clinical isolates of Enterobacteriaceae were collected in Beijing and screened for PMQR genes usingpolymerase chain reaction (PCR). Real-time quantitative PCR was used to study the expression of qnrS.

Results: The rates of qnr and aac(6’)-Ib-cr genes were 7.4% and 8.1%, respectively. The higher basal expression of qnrS was observed in transconjugant strains, which had higher minimum inhibitory concentrations (MICs) of quinolones. Furthermore, qnrS expression levels increased in all three isolates when a quinolone was present.

Conclusions: Our data suggest that the level of qnrS expression was associated with quinolone resistance.

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Published

2014-12-15

How to Cite

Jiang X, Li J, Zhang Y, Yan H, Wang Y, Shi L, Zhou L (2014) Detection of plasmid-mediated quinolone resistance determinants and qnrS expression in Enterobacteriaceae clinical isolates. J Infect Dev Ctries 8:1625–1629. doi: 10.3855/jidc.4617

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Issue

Vol. 8 No. 12: December 2014

Section

Brief Original Articles

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