Evaluation of recent methods versus conventional methods for diagnosis of early-onset neonatal sepsis

  • Ali Kh Al-Zahrani College of Medicine Taif University, Neonatal Intensive Care Unit King Abdulaziz Specialist Hospital, Taif, Saudi Arabia
  • Mabrouk M Ghonaim Faculty of Applied Medical Sciences, Taif University, Saudi Arabia
  • Yousri M Hussein Faculty of Applied Medical Sciences, Taif University, Saudi Arabia
  • Emad M Eed Faculty of Applied Medical Sciences, Taif University, Saudi Arabia
  • Amany S Khalifa Faculty of pharmacy, Taif University, Saudi Arabia
  • Lalia S Dorgham Faculty of Applied Medical Sciences, Taif University, Saudi Arabia
Keywords: neonatal sepsis, PCR, procalcitonin, IL-6, hs-CRP

Abstract

Introduction: Hospital-acquired infections continue to be a major public health problem, especially among neonates. Large proportions of infants are admitted to neonatal intensive care units (NICUs) and receive potent systemic antibiotics while the diagnostic work-up is still in progress. This study aimed to evaluate the recent methods for diagnosing neonatal sepsis (NS) and compare them to conventional diagnostic work-up.

Methodology: The study included 100 neonates divided into three groups: proven early-onset NS, clinical early-onset NS, and negative infectious status. Bacterial DNA was detected in the blood by broad-range 16S rDNA polymerase chain reaction (PCR). Markers for diagnosis of bacterial infection, which includedprocalcitonin (PCT), interleukin-6 (IL-6), and highly sensitive C-reactive protein (hs-CRP), were measured by enzyme-linked immunosorbent assay (ELISA).

Results: Blood culture was positive in 25 cases, while PCR for 16S rDNA was positive in 32 cases. Hs-CRP was significantly elevated in 30 patients in group 1, 35 patients in group 2, and 8 patients in group 3. IL-6 was significantly elevated in 28 patients in group 1, 24 patients in group 2, and 9 patients in group 3. PCT was found to be significantly elevated in 29 patients in group 1, 31 patients in group 2, and 2 patients in group 3.

Conclusions: The16S rDNA PCR assay was more sensitive than blood culture. The combination of markers (hs-CRP, PCT, and IL-6) is better than single markers to diagnose sepsis. PCT had greater diagnostic value than did hs-CRP and IL-6, while IL-6 was better for diagnosis of neonatal infection.

Author Biographies

Ali Kh Al-Zahrani, College of Medicine Taif University, Neonatal Intensive Care Unit King Abdulaziz Specialist Hospital, Taif, Saudi Arabia
Assistant professor at department Pediatrics, College of Medicine Taif University,
Mabrouk M Ghonaim, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia

professor at Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia, andMedical Microbiology & Immunology Department, Faculty of Medicine, Menoufia University , Egypt

Yousri M Hussein, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia
professor at Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia-medical Biochemistry Department, Faculty of Medicine, Zagazig
Emad M Eed, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia
Assitant professor at Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia, andMedical Microbiology & Immunology Department, Faculty of Medicine, Menoufia University , Egypt
Amany S Khalifa, Faculty of pharmacy, Taif University, Saudi Arabia
assitant professor at Medical Microbiology & Immunology Department Faculty of pharmacy, Taif University, Saudi Arabia- Clinical pathology  Department, Faculty of Medicine, Menoufia University, Egypt
Lalia S Dorgham, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia
professor at  Medical Laboratories Department, Faculty of Applied Medical Sciences, Taif University, Saudi Arabia- Community Medicine Department, National Liver Institute, Menoufia University, Egypt
Published
2015-03-15
How to Cite
1.
Al-Zahrani AK, Ghonaim MM, Hussein YM, Eed EM, Khalifa AS, Dorgham LS (2015) Evaluation of recent methods versus conventional methods for diagnosis of early-onset neonatal sepsis. J Infect Dev Ctries 9:388-393. doi: 10.3855/jidc.5950
Section
Original Articles