Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan

  • Shahzad Ali University of Veterinary and Animal Sciences, Lahore, Pakistan
  • Shamim Akhter Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan
  • Heinrich Neubauer Friedrich-Loeffler-Institut, Jena, Germany
  • Falk Melzer Friedrich-Loeffler-Institut, Jena, Germany
  • Iahtasham Khan Friedrich-Loeffler-Institut, Jena, Germany
  • Qurban Ali National Veterinary Laboratory, Park Road, Islamabad, Pakistan
  • Muhammad Irfan National Veterinary Laboratory, Park Road, Islamabad, Pakistan
Keywords: brucellosis, sheep, goat, seroprevalence, PCR, Pakistan

Abstract

Introduction: The objectives of the present study were to determine the seroprevalence and identify the causative agent of brucellosis in small ruminants in Pakistan.

Methodology: A total of 278 serum and 212 milk samples were collected from sheep and goats that had close contact with seropositive bovine herds. Data related to age, sex, location, and breed were collected on the sampling day. Serum and milk samples were initially screened using two different Rose Bengal plate test (RBPT) antigens and a milk ring test (MRT). Seropositive samples were subjected to bacterial isolation and PCR analysis using Brucella genus-specific (bcsp31) and Brucella species-specific (IS711 for Brucella abortus and Brucella melitensis) quantitative real-time polymerase chain reactions (qRT-PCR).

Results: Twenty-four (8.6%) serum samples were positive by RBPT. Twenty (9.4%) animals were positive for Brucella antibodies using MRT. No Brucella isolates were obtained from the examined blood and milk samples. Of the 24 seropositive serum samples, 18 (75%) were positive in the Brucella genus-specific (bcsp31) and Brucella abortus-specific (IS711) qRT-PCR, respectively.

Conclusions: Brucella abortus was identified as causative agent of ovine and caprine brucellosis in Pakistan. Results of this study can be used for the development of an effective control and eradication strategy for brucellosis in livestock, especially small ruminants.

Author Biographies

Shahzad Ali, University of Veterinary and Animal Sciences, Lahore, Pakistan
Department of Zoology, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan
Shamim Akhter, Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan
Associate Professor, Deaprtment of zoology
Heinrich Neubauer, Friedrich-Loeffler-Institut, Jena, Germany

President of Friedrich-Loeffler-Institut, Jena, Germany

and

Head of nstitute of bacterial infection and zoonosis

Falk Melzer, Friedrich-Loeffler-Institut, Jena, Germany
Inchage of Brucellosis research group at institute of bacterial infection and zoonosis
Iahtasham Khan, Friedrich-Loeffler-Institut, Jena, Germany
Visiting scientist at Friedrich-Loeffler-Institut, Jena, Germany
Qurban Ali, National Veterinary Laboratory, Park Road, Islamabad, Pakistan
Director General/Principle Scientific Officer at 

National Veterinary Laboratories, Islamabad, Pakistan

Muhammad Irfan, National Veterinary Laboratory, Park Road, Islamabad, Pakistan
Veterinary Doctor at 

Pir Mehr Ali Shah Arid Agriculture University Rawalpindi, Pakistan

Published
2015-05-18
How to Cite
1.
Ali S, Akhter S, Neubauer H, Melzer F, Khan I, Ali Q, Irfan M (2015) Serological, cultural, and molecular evidence of Brucella infection in small ruminants in Pakistan. J Infect Dev Ctries 9:470-475. doi: 10.3855/jidc.5110
Section
Original Articles