Molecular diagnostics and ITS-based phylogenic analysis of Streptococcus suis serotype 2 in central Vietnam

  • Bach Hoang Nguyen Hue University of Medicine and Pharmacy,University of Hue, Hue, Vietnam
  • Dieu Hong Nu Phan Hue Central Hospital, Hue, Vietnam
  • Hien Xuan Nguyen Hue Central Hospital, Hue, Vietnam
  • An Van Le Hue University of Medicine and Pharmacy,University of Hue, Hue, Vietnam
  • Alberto Alberti University of Sassari, Sassari, Italy
Keywords: Streptococcus suis serotype 2, meningitis, real-time PCR, intergenic spacer (ITS)

Abstract

Introduction: Streptococcus suis (S. suis) serotype 2 has recently become the most prevalent cause of meningitis in adults in many areas of Vietnam. This study provides data on S. suis molecular diagnosis in central Vietnam using a real-time polymerase chain reaction (PCR) assay targeting the S. suis serotype 2 cps2J gene. Additionally, 16S-23S rDNA intragenic spacer (ITS)-based phylogenic analysis of strains isolated from cerebrospinal fluid (CSF) in Thua Thien Hue Province, Vietnam, is presented and discussed.

Methodology: Pathogenic bacteria were isolated from 40 CSF samples, and 18 were identified as S. suis by culture-dependent methods. Capsular serotyping was assessed by real-time PCR. ITS sequences were obtained after traditional PCR and were used in phylogenic analyses.

Results: Pathogenic bacteria were isolated from 36 out of 40 CSF samples. A total of 18 S. suis strains were isolated and assigned to serotype 2 by real-time PCR. One CSF sample, negative when tested by culture-dependent methods, was positive to S. suis serotype 2 by real-time PCR. Pairwise alignments of the 18 ITS sequences did not reveal any variable nucleotide position, and resulted in a single sequence type. Sequences were similar to S. suis serotype 2 reference ITS sequences (> 98.1%), and there was no lack of an ITS spacer region in the isolates.

Conclusions: S. suis serotype 2 is the most prevalent serotype in central Vietnam. Real-time PCR assay proved to be a reliable diagnostic method for early detection of S. suis 2 in CSF samples.

Published
2015-07-04
How to Cite
1.
Nguyen BH, Phan DHN, Nguyen HX, Le AV, Alberti A (2015) Molecular diagnostics and ITS-based phylogenic analysis of Streptococcus suis serotype 2 in central Vietnam. J Infect Dev Ctries 9:624-630. doi: 10.3855/jidc.7027
Section
Original Articles