Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures

  • Fatma Mutlu Sariguzel Kayseri Education and Research Hospital, Kayseri, Turkey
  • Elife Berk Erciyes University Medical School, Kayseri, Turkey
  • Ayse Nedret Koc Erciyes University Medical School, Kayseri, Turkey
  • Hafize Sav Erciyes University Medical School, Kayseri, Turkey
  • Gonca Demir Erciyes University Medical School, Kayseri, Turkey
Keywords: Candida spp., clonal relationship, genotyping, virulence factors

Abstract

Introduction: The aim of study was to investigate the virulence factors of phospholipase, proteinase, esterase production and biofilm formation in Candida species isolated from patients with candidemia, and to assess their relationship with Candida genotypes derived after repetitive sequence-based polymerase chain reaction (rep-PCR) fingerprinting.

Methodology: Fifty-two strains were identified to species level according to conventional methods and sequencing. The DiversiLab system was used for the genotyping. Enzyme activities and biofilm formation were evaluated using microbiological methods.

Results: The 52 strains were identified as follows: 29 C. parapsilosis, 19 C. albicans, 2 C. glabrata, and 2 C. tropicalis. Phospholipase and proteinase activities were observed to have statistically significant differences between C. albicans and non-albicans Candida (NAC) strains (p < 0.05), with C. albicans strains showing higher virulence. Rep-PCR revealed eight major genotypes (A-H).The 19 C. albicans and the 33 non-albicans Candida isolates yielded seven (A-G) and four (A, B, C, H) genotypes, respectively. C. albicans strains were not shown to have a predominant genotype and showed higher phospholipase and proteinase activitiy than did NAC, regardless of genotype. Genotype H (52%) was the predominant genotype for the NAC including 27 C. parapsilosis strains, but the majority of strains showed low virulence.

Conclusions: NAC species were the most common causative agent for candidemia. Genotyping showed low transmission of C. albicans strains, but transmission of C. parapsilosis was high. In candidemia, several Candida virulence factors may be responsible at the same time. However, different genotypes of Candida strains showed different virulence activity.

Author Biographies

Fatma Mutlu Sariguzel, Kayseri Education and Research Hospital, Kayseri, Turkey
Department of Microbiology
Elife Berk, Erciyes University Medical School, Kayseri, Turkey
Department of Microbiology
Ayse Nedret Koc, Erciyes University Medical School, Kayseri, Turkey
Department of Microbiology
Hafize Sav, Erciyes University Medical School, Kayseri, Turkey
Department of Microbiology
Gonca Demir, Erciyes University Medical School, Kayseri, Turkey
Department of Microbiology
Published
2015-08-29
How to Cite
1.
Mutlu Sariguzel F, Berk E, Koc AN, Sav H, Demir G (2015) Investigation of the relationship between virulence factors and genotype of Candida spp. isolated from blood cultures. J Infect Dev Ctries 9:857-864. doi: 10.3855/jidc.5359
Section
Original Articles