Characterization of Leptospira interrogans serovar Pomona isolated from swine in Brazil

  • Fabiana Miraglia Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brasil
  • Luisa Z Moreno Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brasil
  • Zenaide M Morais Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brasil
  • Helio Langoni Laboratório de Diagnóstico em Zoonoses, Unesp, Botucatu, Brasil
  • Fabio H Shimabukuro Instituto Adolfo Lutz, Laboratório Regional de Sorocaba, Sorocaba, Brasil
  • Odir A Dellagostin Centro de Desenvolvimento Tecnológico- Biotecnologia Universidade Federal de Pelotas, Pelotas, Brasil
  • Rudy Hartskeerl WHO/FAO/OIE and National Leptospirosis Reference Centre, KIT Biomedical Research, Royal Tropical Institute, Amsterdam, Netherlands
  • Silvio A Vasconcellos Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brasil
  • Andrea Micke Moreno Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brasil
Keywords: Leptospira interrogans, serovar Pomona, antimicrobial resistance, PFGE, MLST

Abstract

Introduction: Leptospira interrogans swine infection is a cause of serious economic loss and a potential human health hazard. In Brazil, the most common serovars associated with swine infections are Pomona, Icterohaemorrhagie and Tarassovi. Cross-reactions among serovars and the failure of infected animals to seroconvert may complicate the interpretation of serological tests. Molecular methods with better discriminatory powers are useful tools for swine leptospirosis characterization and diagnosis.

Methodology: This study evaluated nine L. interrogans isolates from the States of Sao Paulo and Minas Gerais during different time periods. Isolates from diseased and apparently healthy swine were characterized by microscopic agglutination tests with polyclonal antibodies and were genotyped by VNTR, PFGE and MLST techniques. Broth microdilution was used to determine the minimal inhibitory concentration of the antimicrobials of veterinary interest.

Results: The strains were identified as L. interrogans serogroup Pomona serovar Pomona Genotype A, while MLST grouped all of the isolates in sequence type 37. The PFGE analysis resulted in two pulsotypes with more than 70% similarity, distinguishing serovar Pomona isolates from the serovar Kennewicki reference strain. All of the isolates presented low MIC values to penicillin, ampicillin, ceftiofur and tulathromycin. High MIC values for fluoroquinolones, tiamulin, gentamicin, tetracyclines, neomycin, tilmicosin and sulfas were also observed.

Conclusions: All molecular techniques were concordant in L. interrogans serovar Pomona identification. This serovar may have a different antibiotic susceptibility profile than previously reported for Leptospira isolates.

Author Biography

Andrea Micke Moreno, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Brasil

Professor of Veterinary School and Animal Science

Department of preventive veterinary medicine

Areas of swine health and production, zoonosis, foodborne diseases, molecular epidemiology and antimicrobial resistance.

 

Published
2015-10-29
How to Cite
1.
Miraglia F, Moreno LZ, Morais ZM, Langoni H, Shimabukuro FH, Dellagostin OA, Hartskeerl R, Vasconcellos SA, Moreno AM (2015) Characterization of Leptospira interrogans serovar Pomona isolated from swine in Brazil. J Infect Dev Ctries 9:1054-1061. doi: 10.3855/jidc.5507
Section
Original Articles