Outbreak of Chikungunya virus in the north Caribbean area of Colombia: clinical presentation and phylogenetic analysis

  • Salim Mattar Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • Jorge Miranda Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • Hernando Pinzon Clínica del Niño, Cartagena, Universidad de Cartagena, Colombia
  • Vanesa Tique Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • Amada Bolanos Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • Jose Aponte Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • German Arrieta Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • Marco Gonzalez Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • Katerine Barrios Clínica del Niño, Cartagena, Universidad de Cartagena, Colombia
  • Hector Contreras Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • Jaime Alvarez Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
  • Ader Aleman Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
Keywords: Chikungunya, vector-borne diseases, Culicidae, epidemiology

Abstract

Introduction: The Caribbean area of Colombia has been severely affected by a Chikungunya virus (CHIKV) outbreak since 2014.

Methodology: The study was carried out on 100 patients during a fever outbreak from August to September 2014 in two small rural villages in the northern Caribbean area of Colombia. The molecular assays performed by reverse transcription polymerase chain reaction (RT-PCR) on acute patient sera were collected within one to five days of the appearance of symptoms. Sequence analyses were carried out based on phylogenetic analyses of genes NS1 and E2. For serological assays, 49 (49%) patients at ≥ 6 days of disease onset were tested with NovaLisa Chikungunya IgG/IgM μ-capture enzyme-linked immunosorbent assay (ELISA).

Results: The main signs or symptoms associated with Chikungunya infection were arthralgia of the lower limbs (96%), fever (91%), arthralgia of the upper limbs (85%), rash (64%), and headache (57%). Ninety-four percent (46/49) of patients were positive for either IgM or IgG; the remaining three (6%) patients were seronegative. Viral loads were detected in 25 patients. Based on phylogenetic analysis of NS1 and E2, the characterization of the Colombian CHIKV indicated that it was a strain closely related to the British Virgin Islands strain and to the Asian genotype.

Conclusions: This study shows the phylogenetic and clinical description of CHIKV in Colombia. The main symptoms shown were: arthralgia, fever, and rash. CHIKV sequences detected in Colombian patients were within the Asian genotype and closely related to the British Virgin Islands strain.

Author Biography

Salim Mattar, Universidad de Córdoba, Instituto de Investigaciones Biológicas del Trópico, Montería, Colombia
Director of Instituto de Investigaciones Biologicas del Tropico, Monteria, Colombia, South America.
Published
2015-10-29
How to Cite
1.
Mattar S, Miranda J, Pinzon H, Tique V, Bolanos A, Aponte J, Arrieta G, Gonzalez M, Barrios K, Contreras H, Alvarez J, Aleman A (2015) Outbreak of Chikungunya virus in the north Caribbean area of Colombia: clinical presentation and phylogenetic analysis. J Infect Dev Ctries 9:1126-1132. doi: 10.3855/jidc.6670
Section
Original Articles