Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

  • Rafael Vignoli Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
  • Virginia García-Fulgueiras Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
  • Nicolás F Cordeiro Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
  • Inés Bado Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
  • Verónica Seija Hospital Pasteur, Administración de los Servicios de Salud del Estado, Larravide S/N, Montevideo, Uruguay
  • Paula Aguerrebere Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
  • Gabriel Laguna Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
  • Lucía Araújo Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
  • Cristina Bazet Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
  • Gabriel Gutkind Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
  • José Chabalgoity Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Keywords: virulence genes, ESBL, plasmid-mediated quinolone resistance

Abstract

Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals.

Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction.  ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates.

Results: Thirty-seven isolates harbored blaCTX-M-15 (67.3%), eight blaCTX-M-2 (14.6%), five blaCTX-M-14 (9.1%), three carried both blaCTX-M-2 and blaCTX-M-14, one blaCTX-M-9, and one blaCTX-M-8.  Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6’)Ib-cr as well. Isolates harboring blaCTX-M-2, blaCTX-M-14, blaCTX-M-9,or blaCTX-M-8 were found to be genetically unrelated.

Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.

Author Biography

Rafael Vignoli, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Departamento de Bacteriología y Virología, Instituto de Higiene. Profesor agregado.
Published
2016-01-31
How to Cite
1.
Vignoli R, García-Fulgueiras V, Cordeiro NF, Bado I, Seija V, Aguerrebere P, Laguna G, Araújo L, Bazet C, Gutkind G, Chabalgoity J (2016) Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay. J Infect Dev Ctries 10:43-52. doi: 10.3855/jidc.6918
Section
Original Articles