Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

Rafael Vignoli; Virginia García-Fulgueiras; Nicolás F Cordeiro; Inés Bado; Verónica Seija; Paula Aguerrebere; Gabriel Laguna; Lucía Araújo; Cristina Bazet; Gabriel Gutkind; José Chabalgoity

doi:10.3855/jidc.6918

Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay

Authors

Rafael Vignoli Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Virginia García-Fulgueiras Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Nicolás F Cordeiro Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Inés Bado Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Verónica Seija Hospital Pasteur, Administración de los Servicios de Salud del Estado, Larravide S/N, Montevideo, Uruguay
Paula Aguerrebere Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Gabriel Laguna Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Lucía Araújo Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Cristina Bazet Hospital de Clínicas, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay
Gabriel Gutkind Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
José Chabalgoity Facultad de Medicina, Universidad de la República, Montevideo, Uruguay

DOI:

https://doi.org/10.3855/jidc.6918

Keywords:

virulence genes, ESBL, plasmid-mediated quinolone resistance

Abstract

Introduction: To characterize extended-spectrum β-lactamases (ESBLs) and plasmid-mediated quinolone resistance (PMQR) genes in Escherichia coli isolates obtained from extra-intestinal samples in three Uruguayan hospitals.

Methodology: Fifty-five ESBL-producing E. coli isolates were studied. Virulence genes, ESBLs, and PMQR genes were detected by polymerase chain reaction. ESBL-producing isolates were compared by pulsed-field gel electrophoresis. Multi-locus sequence typing was also performed on 13 selected isolates.

Results: Thirty-seven isolates harbored bla_CTX-M-15 (67.3%), eight bla_CTX-M-2 (14.6%), five bla_CTX-M-14 (9.1%), three carried both bla_CTX-M-2and bla_CTX-M-14, one bla_CTX-M-9, and one bla_CTX-M-8. Among the CTX-M-15 producers, 92% belonged to sequence types ST131 and ST405, and carried aac(6’)Ib-cr as well. Isolates harboring bla_CTX-M-2, bla_CTX-M-14, bla_CTX-M-9,or bla_CTX-M-8 were found to be genetically unrelated.

Conclusions: The successful dissemination of CTX-M-15-producing E.coli isolates seems to be linked to the spreading of high-risk clones and horizontal gene transfer. A trade-off between carrying more antibiotic resistance and less virulence-related genes could partially account for the evolutionary advantages featured by successful clones.

Author Biography

Rafael Vignoli, Facultad de Medicina, Universidad de la República, Montevideo, Uruguay

Departamento de Bacteriología y Virología, Instituto de Higiene. Profesor agregado.

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Published

2016-01-31

How to Cite

Vignoli R, García-Fulgueiras V, Cordeiro NF, Bado I, Seija V, Aguerrebere P, Laguna G, Araújo L, Bazet C, Gutkind G, Chabalgoity J (2016) Extended-spectrum β-lactamases, transferable quinolone resistance, and virulotyping in extra-intestinal E. coli in Uruguay. J Infect Dev Ctries 10:43–52. doi: 10.3855/jidc.6918

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Issue

Vol. 10 No. 01: January 2016

Section

Original Articles

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