spa typing and antimicrobial resistance of Staphylococcus aureus from healthy humans, pigs and dogs in Tanzania

  • Abdul Sekemani Katakweba Sokoine University of Agriculture, Chuo Kikuu Morogoro, Tanzania
  • Amandus Pachificus Muhairwa Sokoine University of Agriculture, Chuo Kikuu Morogoro, Tanzania
  • Carmen Espinosa-Gongora University of Copenhagen, Copenhagen, Denmark
  • Luca Guardabassi University of Copenhagen, Copenhagen, Denmark
  • Madundo M A Mtambo Sokoine University of Agriculture, Chuo Kikuu Morogoro, Tanzania
  • John Elmerdahl Olsen University of Copenhagen, Copenhagen, Denmark
Keywords: Antimicrobials, genotyping, S. aureus, Tanzania

Abstract

Introduction: Staphylococcus aureus is an opportunistic pathogen causing infections in humans and animals. Here we report for the first time the prevalence of nasal carriage, spa typing and antimicrobial resistance of S. aureus in a Tanzanian livestock community.

Methodology: Nasal swabs were taken from 100 humans, 100 pigs and 100 dogs in Morogoro Municipal. Each swab was enriched in Mueller Hinton broth with 6.5% NaCl and subcultured on chromogenic agar for S. aureus detection. Presumptive S. aureus colonies were confirmed to the species level by nuc PCR and analysed by spa typing. Antimicrobial susceptibility patterns were determined by disc diffusion method.

Results: S. aureus was isolated from 22 % of humans, 4 % of pigs and 11 % of dogs. A total of 21 spa types were identified: 13, 7 and 1 in human, dogs, and pigs, respectively. Three spa types (t314, t223 and t084) were shared between humans and dogs. A novel spa type (t10779) was identified in an isolate recovered from a colonized human. Antimicrobials tested revealed resistance to ampicillin in all isolates, moderate resistances to other antimicrobials with tetracycline resistance being the most frequent.

Conclusion: S. aureus carrier frequencies in dogs and humans were within the expected range and low in pigs. The S. aureus spa types circulating in the community were generally not shared by different hosts and majority of types belonged to known clones. Besides ampicillin resistance, moderate levels of antimicrobial resistance were observed irrespective of the host species from which the strains were isolated.

Author Biographies

Abdul Sekemani Katakweba, Sokoine University of Agriculture, Chuo Kikuu Morogoro, Tanzania

Research Fellow in Public Health

Pest Management Centre

 

Amandus Pachificus Muhairwa, Sokoine University of Agriculture, Chuo Kikuu Morogoro, Tanzania

Professor in Poultry Diseases

Department of Veterinary Medicine and Public Health
Carmen Espinosa-Gongora, University of Copenhagen, Copenhagen, Denmark

Post doc in  Veterinary Clinical Microbiology

Department of Veterinary Disease Biology
Luca Guardabassi, University of Copenhagen, Copenhagen, Denmark

Professor in Clinical Microbiology

Department of Veterinary Disease Biology
Madundo M A Mtambo, Sokoine University of Agriculture, Chuo Kikuu Morogoro, Tanzania

Professor in Veterinary Medicine

Department of Veterinary Medicine and Public Health
John Elmerdahl Olsen, University of Copenhagen, Copenhagen, Denmark

Professor in Veterinary Clinical Microbiology

Department of Veterinary Disease Biology
Published
2016-02-28
How to Cite
1.
Katakweba AS, Muhairwa AP, Espinosa-Gongora C, Guardabassi L, Mtambo MMA, Olsen JE (2016) spa typing and antimicrobial resistance of Staphylococcus aureus from healthy humans, pigs and dogs in Tanzania. J Infect Dev Ctries 10:143-148. doi: 10.3855/jidc.6790
Section
Original Articles