Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories

  • Bojana Prunić Scientific Veterinary Institute, Novi Sad, Novi Sad, Republic of Serbia
  • Dubravka Milanov Scientific Veterinary Institute, Novi Sad, Novi Sad, Republic of Serbia
  • Maja Velhner Scientific Veterinary Institute, Novi Sad, Novi Sad, Republic of Serbia
  • Marko Pajić Scientific Veterinary Institute, Novi Sad, Novi Sad, Republic of Serbia
  • Ljiljana Pavlović Institute of Public Health of Serbia Dr. Milan Jovanović Batut, Belgrade, Republic of Serbia
  • Dušan Mišić University of Belgrade, Faculty of Veterinary Medicine, Belgrade, Republic of Serbia
Keywords: feed, PFGE, Salmonella spp., rdar morphotypes

Abstract

Introduction: Novel molecular techniques applied in biotechnology research have provided sound evidence on clonal persistence of distinct serovars of Salmonella in feed factory environments, over long periods of time (months, even years), which can be responsible for repeated in-house contamination of final products. In this study, we examined the possibility of clonal persistence of isolates of three Salmonella serovars that have been repeatedly identified in animal feed samples from three feed factories throughout a two-year period.

Methodology: The isolates Salmonella enterica serovars Tennessee (n = 7), Montevideo (n = 8), and Infantis (n = 4) were tested for genetic diversity using pulsed-field gel electrophoresis (PFGE) and multicellular behavior patterns by applying the Congo red agar test.

Results: SpeI and XbaI macro-restriction profiles indicated that isolates S. Montevideo and S. Infantis were identical, whereas isolates of S. Tennessee demonstrated greater genetic diversity, although the genetic differences did not exceed 10%. All Salmonella serovars demonstrated the ability to produce predominant matrix compounds essential for biofilm formation, curli fimbriae and cellulose.

Conclusions: The identification of identical clones of S. Montevideo and S. Infantis, as well as the minor genetic diversity of S. Tennessee, which have been repeatedly isolated from animal feed in three production plants throughout a two-year period, indirectly suggests the possibility of their persistence in feed factory environments. Their ability to express the key biofilm matrix components further supports this hypothesis.

Author Biographies

Bojana Prunić, Scientific Veterinary Institute, Novi Sad, Novi Sad, Republic of Serbia
Department of Microbiological examination of animal feed
Dubravka Milanov, Scientific Veterinary Institute, Novi Sad, Novi Sad, Republic of Serbia
Department of Microbiological examination of animal feed
Maja Velhner, Scientific Veterinary Institute, Novi Sad, Novi Sad, Republic of Serbia
Department of Clinical Microbiology
Marko Pajić, Scientific Veterinary Institute, Novi Sad, Novi Sad, Republic of Serbia
Department of Epizootiology
Ljiljana Pavlović, Institute of Public Health of Serbia Dr. Milan Jovanović Batut, Belgrade, Republic of Serbia
Department of Microbiology
Dušan Mišić, University of Belgrade, Faculty of Veterinary Medicine, Belgrade, Republic of Serbia
Microbiology and Immunology
Published
2016-06-30
How to Cite
1.
Prunić B, Milanov D, Velhner M, Pajić M, Pavlović L, Mišić D (2016) Clonal persistence of Salmonella enterica serovars Montevideo, Tennessee, and Infantis in feed factories. J Infect Dev Ctries 10:662-666. doi: 10.3855/jidc.7313
Section
Brief Original Articles