Detection of Mycobacterium avium subsp. paratuberculosis in Iranian patients with type 1 diabetes mellitus by PCR and ELISA

  • Sonia Hesam Shariati School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
  • Anoosha Alaei Arad Hospital, Tehran, Iran
  • Rouhollah Keshavarz Razi Vaccine and Serum Research Institute, Karaj, Iran
  • Nader Mosavari Razi Vaccine and Serum Research Institute, Karaj, Iran
  • Ali Rabbani Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
  • Magdalena Niegowska University of Sassari, Sassari, Italy
  • Leonardo A Sechi University of Sassari, Sassari, Italy
  • Mohammad Mehdi Feizabadi School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Keywords: Mycobacterium avium ssp. paratuberculosis, T1DM, ELISA, IS900, PCR

Abstract

Introduction: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis or Johne’s disease in ruminants. Its role in triggering autoimmunity, including type 1 diabetes mellitus (T1DM), has been reported in recent years. Due to the high contamination rate of MAP in Iran’s livestock and the increasing outbreak of T1DM, we investigated this association in a small group of patients with T1DM in Iran.

Methodology: Blood samples of 29 T1DM patients and 29 healthy control subjects were tested through enzyme-linked immunosorbent assay (ELISA) to detect antibodies against MAP3865c and ZnT8 homologous epitopes and the presence of MAP DNA. Blood samples were also cultured in mycobacterial growth indicator tubes and Herrold's egg yolk medium containing mycobactin J.

Results: The results of ELISA showed a significant difference between T1DM patients and healthy group. IS900 was also detected in 51.72% of T1DM patients but in none of the control group. None of the samples grew in culture media.

Conclusions: Due to the presence of MAP DNA and antibodies against MAP peptides in a significant number of T1DM patients compared with healthy control subjects, we may consider MAP as a possible trigger of T1DM in Iran. This indicates that exposure to MAP occurred in the positive subjects. Identifying the sources of contamination and routes of MAP transmission to humans seems necessary to prevent and reduce the burden of MAP infection in Iran.

Author Biographies

Sonia Hesam Shariati, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Master of Medical Microbiology

Department of Microbiology, School of Medicine

 

Rouhollah Keshavarz, Razi Vaccine and Serum Research Institute, Karaj, Iran
PPD Tuberculin Department
Nader Mosavari, Razi Vaccine and Serum Research Institute, Karaj, Iran
PPD Tuberculin Department
Ali Rabbani, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
Growth and Development Research Center
Magdalena Niegowska, University of Sassari, Sassari, Italy
Department of Biomedical Sciences
Leonardo A Sechi, University of Sassari, Sassari, Italy

Full Professor of Microbiology

Department of Biomedical Sciences

Mohammad Mehdi Feizabadi, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Professor in Medical Microbiology

Department of Microbiology, School of Medicine

Published
2016-08-31
How to Cite
1.
Hesam Shariati S, Alaei A, Keshavarz R, Mosavari N, Rabbani A, Niegowska M, Sechi LA, Feizabadi MM (2016) Detection of Mycobacterium avium subsp. paratuberculosis in Iranian patients with type 1 diabetes mellitus by PCR and ELISA. J Infect Dev Ctries 10:857-862. doi: 10.3855/jidc.7473
Section
Original Articles