Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco

  • Bahija Serray Faculty of Sciences Ain-Chock, Casablanca, Morocco
  • Salwa Oufrid Institut Pasteur du Maroc, Casablanca, Morocco
  • Imane Hannaoui Faculty of Sciences and Technics, Sultane Moulay Slimane University, Beni Mellal, Morocco
  • Fatna Bourjilate Institut Pasteur du Maroc, Casablanca, Morocco
  • Nabila Soraa University Hospital Mohamed VI, Marrakech, Morocco
  • Mostafa Mliji Institut Pasteur du Maroc, Casablanca, Morocco
  • Mohammed Sobh Faculty of Sciences Ain-Chock, Casablanca, Morocco
  • Abderrahmane Hammoumi Faculty of Sciences Ain-Chock, Casablanca, Morocco
  • Mohammed Timinouni Institut Pasteur du Maroc, Casablanca, Morocco
  • Mohamed El Azhari Institut Pasteur du Maroc, Casablanca, Morocco
Keywords: adhesion genes, MSCRAMMs, methicillin-resistant Staphylococcus aureus, biofilms

Abstract

Introduction: Infections involving methicillin-resistant Staphylococcus aureus (MRSA) remain a serious threat to hospitalized patients worldwide. MRSA is characterized by recalcitrance to antimicrobial therapy, which is a function not only of widespread antimicrobial resistance, but also the capacity to form biofilms. The present study evaluated the presence of genes encoding adhesion factors and the biofilm-forming capacity in MRSA.

Methodology: In this study, 53 isolates of MRSA, recovered from December 2010 to May 2014 in a mother and child hospital, CHU Mohamed VI in Marrakech, Morocco, were screened for the presence of bap and ica genes associated with biofilm formation, and for bbp, cna, ebpS, eno, fib, fnbA, fnbB, clfA, and clfB genes that encode microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). The biofilm formation assay was performed in 96-well microtiter polystyrene plates. The presence of genes was determined by polymerase chain reaction (PCR).

Results: An association was found between icaD gene detection and biofilm formation; 100% of the strains harbored icaD and produced biofilm. None of the isolates harbored bap or bbp. Furthermore, 96.23% isolates were positive for fnbA, 60.37% for eno, 43.39% for clfA and clfB, 11.32% for cna, 9.34% for ebpS, 5.66% for fib, and 1.89% for fnbA.

Conclusions: Our findings showed that the MRSA carriage in Marrakech children was high. The genetic variations of adhesion genes require further investigation.

Author Biographies

Bahija Serray, Faculty of Sciences Ain-Chock, Casablanca, Morocco
student
Imane Hannaoui, Faculty of Sciences and Technics, Sultane Moulay Slimane University, Beni Mellal, Morocco
student
Published
2016-08-31
How to Cite
1.
Serray B, Oufrid S, Hannaoui I, Bourjilate F, Soraa N, Mliji M, Sobh M, Hammoumi A, Timinouni M, El Azhari M (2016) Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco. J Infect Dev Ctries 10:863-869. doi: 10.3855/jidc.8361
Section
Original Articles