Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness

  • Ageng Wiyatno Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Ungke Antonjaya Eijkman-Oxford Clinical Research Unit, Jakarta, Indonesia
  • Chairin Nisa Ma'roef Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Silvita Fitri Riswari Universitas Padjadjaran, Bandung, Indonesia
  • Hofiya Djauhari Universitas Padjadjaran, Bandung, Indonesia
  • I Made Artika Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Corina Monagin Metabiota, Incorporation, San Francisco, United States
  • Bradley S. Schneider Metabiota, Incorporation, San Francisco, United States
  • Khin Saw Myint Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Bachti Alisjahbana Universitas Padjadjaran, Bandung, Indonesia
  • Dodi Safari Eijkman Institute for Molecular Biology, Jakarta, Indonesia
  • Herman Kosasih Universitas Padjadjaran, Bandung, Indonesia
Keywords: Coxsackievirus, Indonesia, febrile illness

Abstract

Introduction: Coxsackievirus B3 (CVB3) virus has been implicated as the causative agent of various outbreaks of clinical disease, including hand, foot, and mouth diseases, aseptic meningitis, acute myocarditis, and inflammatory cardiomyopathy.

Methodology: Two hundred and nine undiagnosed cryopreserved specimens obtained from factory workers in Bandung, Indonesia, who displayed symptoms of acute febrile illness were gathered. Total RNA was isolated from serum and tested by conventional polymerase chain reaction (PCR) using Enterovirus genus-level primers and confirmed by sequencing. Concurrently, the virus was isolated in LLC-MK2 cells.

Results: CVB3 virus was identified in an archived specimen from a patient who presented with symptoms of fever, headache, myalgia, and nausea. Sequencing results of the VP1 region from both the clinical sample and tissue culture supernatant showed 97% homology to a CVB3 virus isolate from Taiwan. Virus propagation in LLC-MK2 cell culture exhibited severe cytopathic effects two days post-inoculation.

Conclusions: We report the first case of CVB3 from an undifferentiated febrile illness specimen from Indonesia.

Author Biographies

Ageng Wiyatno, Eijkman Institute for Molecular Biology, Jakarta, Indonesia

 

 

Ungke Antonjaya, Eijkman-Oxford Clinical Research Unit, Jakarta, Indonesia

 

 

 

Silvita Fitri Riswari, Universitas Padjadjaran, Bandung, Indonesia

 

 

 

Bachti Alisjahbana, Universitas Padjadjaran, Bandung, Indonesia

 

 

Published
2016-08-31
How to Cite
1.
Wiyatno A, Antonjaya U, Ma’roef CN, Riswari SF, Djauhari H, Artika IM, Monagin C, Schneider BS, Myint KS, Alisjahbana B, Safari D, Kosasih H (2016) Detection and identification of coxsackievirus B3 from sera of an Indonesian patient with undifferentiated febrile illness. J Infect Dev Ctries 10:880-883. doi: 10.3855/jidc.7573
Section
Brief Original Articles