Anti-proteolytic activity of Ganoderma lucidum methanol extract against Pseudomonas aeruginosa

  • Nessma Ahmed El Zawawy Faculty of Science, Tanta University, Tanta, Egypt
  • Sameh Samir Ali Faculty of Science, Tanta University, Tanta, Egypt
Keywords: Ganoderma lucidum, Pseudomonas aeruginosa, multidrug resistance, virulence factor, protease

Abstract

Introduction: Protease enzyme is considered one of the most serious virulence factors produced by extended-spectrum β-lactamase-producing and multidrug-resistant Pseudomonas aeruginosa (ESβLMDRPA) clinical isolates.

Methodology: The antibacterial activity of methanol extract of Ganoderma lucidum fruiting bodies was tested against a protease-producing ESβLMDRPA clinical isolate, showing its mode of action.

Results: The extract showed high antibacterial activity. Its effect on purified protease indicated a reversible non-competitive protease inhibition (kis= 0.45 mg/mL).

Conclusions: The G. lucidum extract could be a promising anti-proteolytic active against ESβLMDRPA. It may form a primary platform for further phytochemical studies and development of new drugs for therapy of skin burn infections.

Author Biography

Nessma Ahmed El Zawawy, Faculty of Science, Tanta University, Tanta, Egypt
Botany department
Published
2016-09-30
How to Cite
1.
El Zawawy NA, Ali SS (2016) Anti-proteolytic activity of Ganoderma lucidum methanol extract against Pseudomonas aeruginosa. J Infect Dev Ctries 10:1020-1024. doi: 10.3855/jidc.6929
Section
Brief Original Articles