Gallbladder microbiota variability in Colombian gallstones patients

  • Ariel Antonio Arteta Faculty of Medicine - CES University, Medellín, Colombia
  • Hernan Carvajal-Restrepo Colombian Institute of Tropical Medicine – CES University, Medellín, Colombia
  • Miryan Margot Sánchez-Jiménez Colombian Institute of Tropical Medicine – CES University, Medellín, Colombia
  • Sergio Diaz-Rodriguez Faculty of Medicine - CES University, Medellín, Colombia
  • Nora Cardona-Castro Faculty of Medicine - CES University, Medellín, Colombia
Keywords: Gallstones, microbiota, enterobacterium, cholecystitis

Abstract

Introduction: Gallbladder stones are a very frequently occurring condition. Despite bile bactericidal activity, many bacteria have been detected inside the gallbladder, and gallstones facilitate their presence. Between 3% and 5% of the patients with Salmonella spp. infection develop the carrier stage, with the bacteria persisting inside the gallbladder, shedding bacteria in their feces without signs of infection. The aim of this study was to isolate bacteria from Colombian patients with gallstones, using standard culturing methods, and to identify Salmonella spp. carriers by molecular techniques.

Methodology: A total of 149 patients (120 female and 29 male) diagnosed with gallstones who underwent cholecystectomy and who did not have symptoms of acute inflammation were included. Gallbladder tissue and bile were cultured and used for DNA extraction and Salmonella spp. hilA gene detection.

Results: Of the 149 patients 28 (19%) had positive cultures. Twenty-one (75%) patients with positive cultures were from Medellin’s metropolitan area. In this geographical location, the most frequent isolations were Pseudomonas spp. (38%), Klebsiella spp. (23%), and Proteus spp. (9%) in addition to unique cases of other bacteria. In Apartado, the isolates found were Enterobacter cloacae (50%), Raoultella terrigena (32%), and both Enterobacter cloacae and Raoultella terrigena were isolated in one (18%) male patient. Five (3.3%) of the 149 patients had positive polymerase chain reaction (PCR) results for the hilA gene of Salmonella spp., all of whom were female and residents of the Medellín metropolitan area.

Conclusions: The gallbladder microbiota variability found could be related to geographical, ethnic, and environmental conditions.

Author Biographies

Ariel Antonio Arteta, Faculty of Medicine - CES University, Medellín, Colombia
Faculty of Medicine, MD. Pathologist.
Hernan Carvajal-Restrepo, Colombian Institute of Tropical Medicine – CES University, Medellín, Colombia
Microbiologist. Colombian Institute of Tropical Medicine
Miryan Margot Sánchez-Jiménez, Colombian Institute of Tropical Medicine – CES University, Medellín, Colombia
MSc. PhD. Colombian Institute of Tropical Medicine
Sergio Diaz-Rodriguez, Faculty of Medicine - CES University, Medellín, Colombia
MD. Surgeon. Faculty of Medicine
Nora Cardona-Castro, Faculty of Medicine - CES University, Medellín, Colombia

MD. MSc. PhD Researcher.

Full Professor

Colombian Institute of Tropical Medicine, Faculty of Medicine

Published
2017-03-31
How to Cite
1.
Arteta AA, Carvajal-Restrepo H, Sánchez-Jiménez MM, Diaz-Rodriguez S, Cardona-Castro N (2017) Gallbladder microbiota variability in Colombian gallstones patients. J Infect Dev Ctries 11:255-260. doi: 10.3855/jidc.8258
Section
Original Articles