Geographical distribution of Brucella melitensis inferred from rpoB gene variation

Kim-Kee Tan; Yung-Chie Tan; Li-Yen Chang; Kok Wei Lee; Siti Sarah Nor'e; Wai-Yan Yee; Chee-Choong Hoh; Sazaly AbuBakar

doi:10.3855/jidc.7598

Geographical distribution of Brucella melitensis inferred from rpoB gene variation

Authors

Kim-Kee Tan University of Malaya, Kuala Lumpur, Malaysia
Yung-Chie Tan Codon Genomics S/B, Seri Kembangan, Malaysia
Li-Yen Chang University of Malaya, Kuala Lumpur, Malaysia
Kok Wei Lee Codon Genomics S/B, Seri Kembangan, Malaysia
Siti Sarah Nor'e University of Malaya, Kuala Lumpur, Malaysia
Wai-Yan Yee Codon Genomics S/B, Seri Kembangan, Malaysia
Chee-Choong Hoh Codon Genomics S/B, Seri Kembangan, Malaysia
Sazaly AbuBakar University of Malaya, Kuala Lumpur, Malaysia

DOI:

https://doi.org/10.3855/jidc.7598

Keywords:

Brucella, rpoB, SNPs

Abstract

Introduction: Currently available tests have limitations for the identification of Brucella species and strains, and their genetic lineage. The genome sequence of the rpoB gene encoding the β-subunit of DNA-dependent RNA polymerase was investigated for its use in genotyping Brucella melitensis.

Methodology: Complete rpoB gene sequences of globally distributed Brucella melitensis strains were analyzed. Single nucleotides polymorphisms (SNPs) of the rpoB gene sequences were identified and used to type Brucella melitensis strains.

Results: Six DNA polymorphisms were identified, of which two (nucleotides 3201 and 558) were novel. Analysis of the geographical distribution of the strains revealed a spatial clustering pattern with rpoB type 1 representing European and American strains, rpoB type 2 representing European, African, and Asian strains, rpoB type 3 representing Mediterranean strains, and rpoB type 4 representing African (C3201T) and European (C3201T/T558A) strains.

Conclusions: We report the discovery of two novel SNPs of rpoB gene that can serve as useful markers for epidemiology and geographical tracking of B. melitensis.

Author Biographies

Kim-Kee Tan, University of Malaya, Kuala Lumpur, Malaysia

Tropical Infectious Diseases Research and Education Center (TIDREC)

Yung-Chie Tan, Codon Genomics S/B, Seri Kembangan, Malaysia

Department of Science and Technology

Li-Yen Chang, University of Malaya, Kuala Lumpur, Malaysia

Tropical Infectious Diseases Research and Education Center (TIDREC)

Kok Wei Lee, Codon Genomics S/B, Seri Kembangan, Malaysia

Department of Science and Technology

Siti Sarah Nor'e, University of Malaya, Kuala Lumpur, Malaysia

Tropical Infectious Diseases Research and Education Center (TIDREC)

Wai-Yan Yee, Codon Genomics S/B, Seri Kembangan, Malaysia

Department of Science and Technology

Chee-Choong Hoh, Codon Genomics S/B, Seri Kembangan, Malaysia

Department of Science and Technology

Sazaly AbuBakar, University of Malaya, Kuala Lumpur, Malaysia

Tropical Infectious Diseases Research and Education Center (TIDREC)

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Published

2017-06-01

How to Cite

Tan K-K, Tan Y-C, Chang L-Y, Lee KW, Nor’e SS, Yee W-Y, Hoh C-C, AbuBakar S (2017) Geographical distribution of Brucella melitensis inferred from rpoB gene variation. J Infect Dev Ctries 11:420–425. doi: 10.3855/jidc.7598

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Issue

Vol. 11 No. 05: May 2017

Section

Brief Original Articles

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