Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria

  • Tolulope Funbi Jolaiya Department of Microbiology, University of Lagos, Akoka, Lagos state, Nigeria
  • Muinah Adenike Fowora Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, Nigeria
  • Charles Onyekwere Department of Medicine, Lagos State University Teaching Hospital, Ikeja, Lagos state, Nigeria
  • Rose Ugiagbe Department of Medicine, University of Benin Teaching Hospital, Benin, Edo state, Nigeria
  • Ifeanyi Ifeoma Agbo Department of Medicine, University of Benin Teaching Hospital, Benin, Edo state, Nigeria
  • Olufunmilayo Lesi Department of Medicine, Lagos University Teaching Hospital, Idi-Araba, Lagos state, Nigeria
  • Dennis Amojuayi Ndububa Department of Medicine, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Osun state, Nigeria
  • Olusegun Adekanle Department of Medicine, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Osun state, Nigeria
  • Henry Akum Njom Department of Biochemistry and Microbiology, University of Fort Hare, Alice, Eastern cape, South Africa
  • Ayodeji Idowu Department of BiDepartment of Biochemistry and Microbiology, University of Fort Hare, Alice, Eastern cape, South Africaochemistry and Microbiology, University of Fort Hare, South Africa
  • Isaac Adeyemi Adeleye Department of Microbiology, University of Lagos, Akoka, Lagos state, Nigeria
  • Moses Bamidele Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, Nigeria
  • Favor Ndidiamaka Ngoka Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, Nigeria
  • Pia Fiona Palamides Chair of Medical Microbiology and Hospital Epidemiology, Max von Pettenkofer Institute, Faculty of Medicine, LMU Munich, Germany
  • Stella Ifeanyi Smith Molecular Biology and Biotechnology Department, Nigerian Institute of Medical Research, Yaba, Lagos state, Nigeria
Keywords: Helicobacter pylori, Sigma factors, Plasticity region genes, DupA, Transcription, Pathology

Abstract

Introduction: Helicobacter pylori is a principal cause of gastric cancer. The aim of this study was to determine the prevalence and contribution of duodenal ulcer promoting gene A (dupA), the plasticity region genes and sigma factors in relation to their pathological expression of H. pylori infections in the Nigerian population.

Methodology: Polymerase Chain Reaction was used to analyze a total of forty-nine H. pylori strains isolated from patients attending various endoscopic units in tertiary hospitals in Nigeria for complete dupA (G27 variant), jhp0917, jhp0918, other plasticity region genes jhp 914/917, jhp0914, jhp0940 and sigma factors.

Results: PCR results indicated that the prevalence of complete dupA (G27 variants), jhp0917, jhp0918 and other plasticity region genes jhp0914, jhp0914/0917 and jhp0940 in the H. pylori strains were 4%, 53%, 88%, 73%, 12% and 0% respectively. The prevalence values of the sigma factors were 96%, 92%, 80% for rpoN,  fliA and rpoD respectively. However, the endoscopic findings showed that erosion, normal mucosal, ulcer, hyperaemic stomach, mucosal atrophy and oedematous stomach in the patients where the H. pylori strains were isolated were 40.8%, 32.7%, 10.2%, 8.2%, 2.0% and 6.1% respectively. There was significant association between jhp0917, jhp914/917 and G27 variant and the endoscopic findings, while other plasticity genes showed no association with the endoscopic findings.

Conclusion: These results suggest that the presence of jhp0917, jhp0914/917 and G27 variant could be used as marker to predict the pathological effect of severity in Nigeria patients with H. pylori infection.

Published
2020-02-29
How to Cite
1.
Jolaiya TF, Fowora MA, Onyekwere C, Ugiagbe R, Agbo II, Lesi O, Ndububa DA, Adekanle O, Njom HA, Idowu A, Adeleye IA, Bamidele M, Ngoka FN, Palamides PF, Smith SI (2020) Duodenal ulcer promoting gene (DupA), plasticity region genes and sigma factors in H. pyloristrains from Nigeria. J Infect Dev Ctries 14:162-168. doi: 10.3855/jidc.11746
Section
Original Articles