Development of a LAMP method for detection of carbapenem-resistant Acinetobacter baumannii during a hospital outbreak

  • Carolina Garciglia Mercado Centro de Investigaciones Biológicas del Noroeste S.C., La Paz Baja California Sur, México
  • Ramon Gaxiola Robles Centro de Investigaciones Biológicas del Noroeste S.C., La Paz Baja California Sur, México
  • Felipe Ascencio Centro de Investigaciones Biológicas del Noroeste S.C., La Paz Baja California Sur, México
  • Jesus Silva-Sanchez Instituto Nacional de Salud Pública, Centro de Investigación Sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, México
  • Maria Teresa Estrada-Garcia CINVESTAV-IPN, Departamento de Biomedicina Molecular, México DF
  • Gracia Gomez-Anduro Centro de Investigaciones Biológicas del Noroeste S.C., La Paz Baja California Sur, México
Keywords: Acinetobacter baumannii, Loop mediated isothermal amplification, Carbapenem resistance

Abstract

Introduction: Carbapenem-resistant A. baumannii (CRAB) represents a public health threat increasing worldwide. We assess the suitability of a loop-mediated isothermal amplification (LAMP) method for on-site screening of CRAB in a hospital facility.

Methodology: A set of six primers were designed for recognizing eight distinct sequences on six targets: blaOXA-23-like, blaOXA-24-like, blaOXA-51-like, blaOXA-58-like, blaIMP, and blaVIM. A LAMP method was developed, optimized and evaluated for the identification of CRAB in thirty-three environmental samples from an outbreak in an Intensive Care Unit (ICU) facility.

Results: The sensitivity of the LAMP assay for the detection of A. baumannii was ten-fold higher than the PCR assay (1.0 ng.µL-1). The LAMP assays showed a higher detection rate for CRAB samples and robust diagnosis performance in comparison to a conventional PCR, with clinical sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of 100% for blaOXA-23-like, blaOXA-51-like and blaVIM.

Conclusions: The developed LAMP assays are powerful tools that can be useful in on-site screening of CRAB causing local outbreaks in clinics and hospitals facilities where costs and equipment restraints are imperative.

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Published
2020-05-31
How to Cite
1.
Garciglia MercadoC, Gaxiola RoblesR, Ascencio F, Silva-SanchezJ, Estrada-GarciaMT, Gomez-AnduroG (2020) Development of a LAMP method for detection of carbapenem-resistant Acinetobacter baumannii during a hospital outbreak. J Infect Dev Ctries 14:494-501. doi: 10.3855/jidc.11692
Section
Original Articles

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