Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis

  • Monira A Selim Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Eman M Fawzy Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Eman M Abd El-Rahman Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Reem S Abdel Hady Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Mohamed S Badr Department of Obstetrics and Gynecology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
  • Enas F Abdel Hamed Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Keywords: Trichomonas Vaginalis, Allium sativum, Nigella sativa

Abstract

Introduction: Trichomoniasis is a worldwide sexually transmitted disease caused by Trichomonas vaginalis. It inflicts severe complications to the human genitourinary system. The devastating negative effects and the emergence of resistance to common medication impose the search for safer and effective alternatives. This research aimed to investigate the effect of the Allium sativum, Nigella sativa crude extracts (NsCE) and the combination between their most effective doses with metronidazole.

Methodology: Vaginal swabs were obtained from symptomatic patients, and cultured on Diamond's medium. Assessment of various concentrations of these herbs at different follow-up periods was done by counting the number of dead T. vaginalis trophozoites using the hemocytometer and trypan blue staining. Transmission electron microscope study was done.

Results: NsCE 9 mg/mL yielded the highest lethal effect on T. vaginalis trophozoites after 72 hours, compared with metronidazole. Combination of NsCE 9 mg/mL and metronidazole 50 µg/mL gave the best result. Additionally, Tomex90 µg/mL, represents a tolerable effect after 72 hours, but metronidazole 100 µg/mL still has higher effect. These results were confirmed by the ultrastructural changes observed in T. vaginalis trophozoites, signifying severe damage of nucleus and cytoplasm with large vacuolization and cell membrane defects.

Conclusions: NsCE is a promising anti-Trichomonas especially its combination with metronidazole which showed a high synergistic effect.

Author Biographies

Eman M Fawzy, Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Lecturer of Medical Parasitology

Eman M Abd El-Rahman, Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Lecturer of Medical Parasitology

Reem S Abdel Hady, Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

demonstrator of Medical Parasitology

Mohamed S Badr, Department of Obstetrics and Gynecology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

lecturer of Obstetrics and Gynecology

Enas F Abdel Hamed, Department of Medical Parasitology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

Lecturer of Medical Parasitology

Published
2020-07-31
How to Cite
1.
Selim MA, Fawzy EM, Abd El-Rahman EM, Abdel Hady RS, Badr MS, Abdel Hamed EF (2020) Evaluation of the effect of some medicinal plants on cultured Trichomonas Vaginalis. J Infect Dev Ctries 14:793-799. doi: 10.3855/jidc.11580
Section
Brief Original Articles