Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients

  • Juan Manuel Bello-López División de Investigación, Hospital Juárez de México, Ciudad de México, México https://orcid.org/0000-0002-9957-3807
  • Carlos Sanchez- Garibay Departamento de Neuropatología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México, México https://orcid.org/0000-0002-0637-5239
  • Gabriela Ibáñez-Cervantes División de Investigación, Hospital Juárez de México, Ciudad de México, México https://orcid.org/0000-0002-7969-0942
  • Gregorio León-García Hospital de la Mujer, Ciudad de México, México
  • Luis Uriel Gonzalez-Avila Laboratorio de Investigación Clínica y Ambiental, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México https://orcid.org/0000-0002-2135-6538
  • Cecilia Hernández-Cortez Laboratorio Bioquímica Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México https://orcid.org/0000-0001-5474-7642
  • Patricia Arzate Barbosa Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Autónoma de México, Ciudad de México, México
  • Graciela Castro-Escarpulli Laboratorio de Investigación Clínica y Ambiental, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México
Keywords: Aeromonas spp., antimicrobial resistance, class 1 integrons, β-Lactamases

Abstract

Introduction: Intestinal and extraintestinal infections by Aeromonas spp., remain controversial, due to the existence of healthy carriers of Aeromonas spp. In children under five years old, the diarrhea of infectious origin constitutes the second cause of mortality and remains a major concern for public health. The aim of this work was to detect the pheno/genotype of β-lactamases and class 1 integrons in Aeromonas spp., strains isolated from pediatric patients in a tertiary referral hospital in Mexico.

Methodology: Sixty-six strains of Aeromonas spp., were isolated from clinical samples of pediatric origin and were identified by RFLP-PCR 16S rRNA. Resistance phenotype according to CLSI, genetic and phenotypic detection of extended-spectrum β-lactamases (ESBL) and metallo-b-lactamases (MBL) was performed. Finally, characterization of class 1 integrons was performed.

Results: Aeromonas spp., strains of diarrheic origin were more predominant. A wide heterogeneity was detected, where A. caviae was the predominant specie. Second-generation cephalosporins, fluoroquinolones, and nitrofurans had best antimicrobial activity; moreover, antibiotics of the β-lactamic and lincosamides families showed lower inhibitory activity. Phenotypically, prevalences of 4.55% and 3.03% were detected for MBL (intestinal origin) and ESBL (extraintestinal origin), respectively. blaIMIS-cphA and blaTEM-1 genes, and nineteen class 1 integrons carrying two variants of cassettes corresponding to adenylyl transferases (aadA), and dihydrofolate reductases (dfrA). Monogenic array with aadA1 cassette was predominantly.

Conclusions: ESBL and class 1 integrons, in Aeromonas collected from pediatric patients, determines a major detection challenge for the clinical microbiology laboratory and represents a remarkable epidemiological risk of nosocomial spread of multidrug-resistant determinants.

Author Biographies

Juan Manuel Bello-López, División de Investigación, Hospital Juárez de México, Ciudad de México, México

Researcher in Medical Sciences (Dirección de Investigación)

Carlos Sanchez- Garibay, Departamento de Neuropatología, Instituto Nacional de Neurología y Neurocirugía Manuel Velasco Suárez, Ciudad de México, México

Researcher in Medical Sciences

Gabriela Ibáñez-Cervantes, División de Investigación, Hospital Juárez de México, Ciudad de México, México

PROFESOR INVITADO (POSGRADO)

Gregorio León-García, Hospital de la Mujer, Ciudad de México, México

Profesor Posgrado

Luis Uriel Gonzalez-Avila, Laboratorio de Investigación Clínica y Ambiental, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México

Professor (Microbiología)

Cecilia Hernández-Cortez, Laboratorio Bioquímica Microbiana, Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México

Research professor (Microbiology)

Patricia Arzate Barbosa, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Autónoma de México, Ciudad de México, México

Research professor (Microbiology)

Published
2020-10-31
How to Cite
1.
Bello-López JM, Sanchez- Garibay C, Ibáñez-Cervantes G, León-García G, Gonzalez-Avila LU, Hernández-Cortez C, Arzate Barbosa P, Castro-Escarpulli G (2020) Genetic and phenotypic determinants of resistance to antibiotics in Aeromonas spp., strains isolated from pediatric patients. J Infect Dev Ctries 14:1146-1154. doi: 10.3855/jidc.12966
Section
Original Articles