Genetic diversity of Mycobacterium tuberculosis using 24-locus MIRU-VNTR typing and Spoligotyping in Upper Myanmar

  • Waing Waing Moe Sann Medical Technology Program, Faculty of Associated medical Sciences, Khon Kaen University, Khon Kaen,Thailand
  • Wises Namwat Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
  • Kiatichai Faksri Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
  • Thyn Lei Swe National TB Control Program, Yangon, Myanmar
  • Kyi Kyi Swe National TB Control Program, Upper Myanmar TB Reference Laboratory, Mandalay Region, Myanmar
  • Thandar Thwin National TB Control Program, Upper Myanmar TB Reference Laboratory, Mandalay Region, Myanmar
  • Arunnee Sangka Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand
Keywords: MIRU-VNTR, spoligotyping, Beijing, Lineages, HGDI

Abstract

Introduction: MIRU-VNTR typing and Spoligotyping are the useful molecular tools for TB epidemiology study. Information regarding genetic diversity and tuberculosis (TB) transmission in Upper Myanmar only is scares.

Methodology: We determined the genetic diversity of Mycobacterium tuberculosis (Mtb) and TB transmission from Upper Myanmar TB Reference Laboratory, Mandalay Region, including Mandalay (72), Shan (22), Magway (15), Sagaing (13), Nay Pyi Taw (8), Kachin (7), Chin (2) and Kayah (1). One hundred and forty Mtb isolates were genotyped using 24-locus MIRU-VNTR typing and spoligotyping. Lineage classification and TB transmission analysis were performed.

Results: 24-locus MIRU-VNTR typing identified 135 unique profiles and two clusters compared to 35 spoligotyping profiles which contained 12 clusters and 23 unique isolates, Beijing (n=100, 71.4%) was found to be prominent lineage by combine two methods. The expected proportion attributable to recent transmission based on clustering rate was 2.1%. One cluster case was more likely to be in MDR patient.

Conclusions: Our findings showed Beijing genotypes were dominant in Upper Myanmar. The usage and analysis of 24-locus MIRU-VNTR typing might prove useful for our broader understanding of TB outbreaks and epidemiology than spoligotyping. The genotypic pattern of this combined method suggests that the lower transmission rate may be due to a higher possibility of reactivation cases in Upper Myanmar.

Published
2020-11-30
How to Cite
1.
Moe Sann WW, Namwat W, Faksri K, Swe TL, Swe KK, Thwin T, Sangka A (2020) Genetic diversity of Mycobacterium tuberculosis using 24-locus MIRU-VNTR typing and Spoligotyping in Upper Myanmar. J Infect Dev Ctries 14:1296-1305. doi: 10.3855/jidc.12998
Section
Original Articles