Genetic diversity of Mycobacterium tuberculosis using 24-locus MIRU-VNTR typing and Spoligotyping in Upper Myanmar

Authors

  • Waing Waing Moe Sann Medical Technology Program, Faculty of Associated medical Sciences, Khon Kaen University, Khon Kaen,Thailand
  • Wises Namwat Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
  • Kiatichai Faksri Department of Microbiology, Faculty of Medicine, Khon Kaen University, Thailand
  • Thyn Lei Swe National TB Control Program, Yangon, Myanmar
  • Kyi Kyi Swe National TB Control Program, Upper Myanmar TB Reference Laboratory, Mandalay Region, Myanmar
  • Thandar Thwin National TB Control Program, Upper Myanmar TB Reference Laboratory, Mandalay Region, Myanmar
  • Arunnee Sangka Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand

DOI:

https://doi.org/10.3855/jidc.12998

Keywords:

MIRU-VNTR, spoligotyping, Beijing, Lineages, HGDI

Abstract

Introduction: MIRU-VNTR typing and Spoligotyping are the useful molecular tools for TB epidemiology study. Information regarding genetic diversity and tuberculosis (TB) transmission in Upper Myanmar only is scares.

Methodology: We determined the genetic diversity of Mycobacterium tuberculosis (Mtb) and TB transmission from Upper Myanmar TB Reference Laboratory, Mandalay Region, including Mandalay (72), Shan (22), Magway (15), Sagaing (13), Nay Pyi Taw (8), Kachin (7), Chin (2) and Kayah (1). One hundred and forty Mtb isolates were genotyped using 24-locus MIRU-VNTR typing and spoligotyping. Lineage classification and TB transmission analysis were performed.

Results: 24-locus MIRU-VNTR typing identified 135 unique profiles and two clusters compared to 35 spoligotyping profiles which contained 12 clusters and 23 unique isolates, Beijing (n=100, 71.4%) was found to be prominent lineage by combine two methods. The expected proportion attributable to recent transmission based on clustering rate was 2.1%. One cluster case was more likely to be in MDR patient.

Conclusions: Our findings showed Beijing genotypes were dominant in Upper Myanmar. The usage and analysis of 24-locus MIRU-VNTR typing might prove useful for our broader understanding of TB outbreaks and epidemiology than spoligotyping. The genotypic pattern of this combined method suggests that the lower transmission rate may be due to a higher possibility of reactivation cases in Upper Myanmar.

Downloads

Published

2020-11-30

How to Cite

1.
Moe Sann WW, Namwat W, Faksri K, Swe TL, Swe KK, Thwin T, Sangka A (2020) Genetic diversity of Mycobacterium tuberculosis using 24-locus MIRU-VNTR typing and Spoligotyping in Upper Myanmar. J Infect Dev Ctries 14:1296–1305. doi: 10.3855/jidc.12998

Issue

Vol. 14 No. 11: November 2020

Section

Original Articles

License

Authors who publish with this journal agree to the following terms:

  1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  3. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Crossref
Scopus
Google Scholar
Europe PMC