Frequency of Giardia duodenalis infection and its genetic variability in dogs in Cuiabá, Midwest Brazil

  • Yolanda Paim Arruda Trevisan Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Arleana do Bom Parto Ferreira de Almeida Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Luciano Nakazato Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Thabata dos Anjos Pacheco Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Jéssica Iglesias de Souza Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Darlan Henrique Canei Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Mariana Elisa Pereira Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Maerle Oliveira Maia Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Richard Campos Pacheco Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
  • Val´éria Régia Franco Sousa Veterinary Hospital, Faculty of Veterinary Medicine, Federal University of Mato Grosso, Boa Esperança, Cuiabá, Brazil
Keywords: molecular characterization, multilocus genotyping, waterborne

Abstract

Introduction: Giardia duodenalis, a unicellular, eukaryotic, and flagellated protozoan, presents two evolutionary forms in its life cycle, namely, trophozoites and cysts. It causes diarrhea in humans, dogs, cats, rodents, and ungulates. Despite being morphologically similar, the isolates of G. duodenalis are genetically diverse, affecting the stability and unanimity of taxonomic classification. Since different Giardia assemblages may occur within one isolate, multilocus genotyping is recommended for the genetic identification.

Methodology: To determine the frequency of G. duodenalis infections in domiciled dogs in Cuiabá Municipality (State of Mato Grosso, Midwestern Brazil) and characterize its genetic variability, fecal samples were collected from 147 dogs.

Results: Overall, 6.8% (10/147) of the samples presented cysts of G. duodenalis, which sequencing and genotypic characterization using tpi and gluD revealed assemblages C and A, genetic grouping of G. duodenalis. Only three samples amplified by tpi and one sample amplified by gluD.

Conclusions: The risk factors age, gender, breed, diet and the presence of other dogs in the same house were not correlationated with giardiasis. The host-specific and zoonotic genotype warns of the risk of inter and intraspecies transmission and it provides, for the first time, information about genetic characterization of G. duodenalis isolates in dogs in Cuiabá, Midwest region of Brazil.

Published
2020-12-31
How to Cite
1.
Paim Arruda Trevisan Y, do Bom Parto Ferreira de Almeida A, Nakazato L, dos Anjos Pacheco T, Iglesias de Souza J, Henrique Canei D, Elisa Pereira M, Oliveira Maia M, Campos Pacheco R, Régia Franco Sousa V (2020) Frequency of Giardia duodenalis infection and its genetic variability in dogs in Cuiabá, Midwest Brazil. J Infect Dev Ctries 14:1431-1436. doi: 10.3855/jidc.13095
Section
Original Articles