Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs

  • Oswaldo M Torres-Chable División Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México
  • Ligia G Brito-Argaez Laboratorio de Bioquímica y Biología Molecular, Centro de Investigaciones Científicas de Yucatán (CICY), Mérida, Yucatán, México
  • Ignacio R Islas-Flores Laboratorio de Bioquímica y Biología Molecular, Centro de Investigaciones Científicas de Yucatán (CICY), Mérida, Yucatán, México
  • Claudia V Zaragoza-Vera División Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México
  • Maritza Zaragoza-Vera División Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México
  • Guadalupe Arjona-Jimenez División Académica de Ciencias Agropecuarias, Laboratorio de Enfermedades Tropicales y Transmitidas por Vectores, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco
  • Carlos M Baak-Baak Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México
  • Nohemi Cigarroa-Toledo Laboratorio de Biología Celular, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México
  • Roberto Gonzalez-Garduño Unidad Regional Universitaria Sursureste, Universidad Autónoma Chapingo, Teapa, Tabasco, México
  • Carlos I Machain-Williams Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México
  • Julian E Garcia-Rejon Laboratorio de Arbovirología, Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán, Mérida, Yucatán, México
Keywords: Dirofilaria, filariasis, zoonotic, immunoreactivity, tropical diseases

Abstract

Introduction: Dirofilaria immitis is a nematode that affects human health in several countries of the world. This study was conducted to examine whether serum samples from the owners of microfilaremic dogs present immunoreactivity to parasite proteins.

Methodology: Eight serum samples from the owners of microfilaremic dogs were examined. Total proteins were extracted from adult worms and 12% SDS-PAGE was performed. The gel was electroblotted to a nitrocellulose membrane, and a Western blot (WB) was performed. Reactive bands of 22, 33, 39, 49, and 63 kDa in WB were excised from the gel and analyzed by mass spectrometry (MS).

Results: The MS results showed the presence of 10 different proteins of D. immitis recognized by the human serum samples.

Conclusions: These results indicate that in endemic areas of D. immitis, owners of infected dogs recognize specific proteins of the parasite, suggesting a possible infection.

Published
2020-12-31
How to Cite
1.
Torres-Chable OM, Brito-Argaez LG, Islas-Flores IR, Zaragoza-Vera CV, Zaragoza-Vera M, Arjona-Jimenez G, Baak-Baak CM, Cigarroa-Toledo N, Gonzalez-Garduño R, Machain-Williams CI, Garcia-Rejon JE (2020) Dirofilaria immitis proteins recognized by antibodies from individuals living with microfilaremic dogs. J Infect Dev Ctries 14:1442-1447. doi: 10.3855/jidc.12711
Section
Original Articles