Clonal dispersion of Acinetobacter baumannii in an intensive care unit designed to patients COVID-19

  • Emilio Mariano Durán-Manuel Hospital Juárez de México, Ciudad de México, México
  • Clemente Cruz-Cruz Hospital Juárez de México, Ciudad de México, México
  • Gabriela Ibáñez-Cervantes Hospital Juárez de México, Ciudad de México, México
  • Juan Carlos Bravata-Alcantará Hospital Juárez de México, Ciudad de México, México
  • Oscar Sosa-Hernández Hospital General de Zona 2 Benigno Arriaga, Tequisquiapan, San Luis Potosí, México
  • Laura Delgado-Balbuena Hospital Juárez de México, Ciudad de México, México
  • Gregorio León-García Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México
  • Iliana Alejandra Cortés-Ortíz Hospital Juárez de México, Ciudad de México, México
  • Monica Alethia Cureño-Díaz Hospital Juárez de México, Ciudad de México, México
  • Graciela Castro-Escarpulli Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México
  • Juan Manuel Vélez-Reséndiz Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México
  • Juan Manuel Bello-López Hospital Juárez de México, Ciudad de México, México
Keywords: ESKAPE bacteria, clonal dispersion, Acinetobacter baumannii, intensive care unit, COVID-19

Abstract

Introduction: SARS-CoV2 pandemic marks the need to pay attention to bacterial pathogens that can complicate the hospital stay of patients in the intensive care unit (ICU). ESKAPE bacteria which includes Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter cloacae are considered the most important, because of their close relationship with the development of ventilator-associated pneumonia (VAP). The aim of this work was to identify and characterize ESKAPE bacteria and to detect their possible clonal spread in medical devices, patients, and medical personnel of the ICU for COVID-19 patients of the Hospital Juarez de Mexico.

Methodology: Genetic identification of ESKAPE bacteria was performed by analyzing the 16S rRNA gene. Resistance assays were performed according to the CLSI guidelines. Assembly of AdeABCRS operon and inhibition assays of pumps efflux in Acinetobacter baumannii isolates were performed. Associated gene involved in biofilm formation (icaA) was performed in isolates belonging to the Staphylococcus genus. Finally, typing by ERIC-PCR and characterization of mobile genetic element SCCmec were done.

Results: Heterogeneous distribution of ESKAPE and non-ESKAPE bacteria was detected in various medical devices, patients, and medical personnel. Acinetobacter baumannii and Staphylococcus aureus were the predominant ESKAPE members. The analysis of intergenic regions revealed an important clonal distribution of A. baumannii (AdeABCRS+). Genotyping of SCCmec mobile genetic elements and the icaA gene showed that there is no clonal distribution of S. aureus.

Conclusions: Clonal spread of A. baumannii (AdeABCRS+) highlights the importance of adopting good practices for equipment disinfection, surfaces and management of COVID-19 patients.

Author Biographies

Emilio Mariano Durán-Manuel, Hospital Juárez de México, Ciudad de México, México

Master in Sciences student

Clemente Cruz-Cruz, Hospital Juárez de México, Ciudad de México, México

Master in Sciences student

Gabriela Ibáñez-Cervantes, Hospital Juárez de México, Ciudad de México, México

Researcher in Medical Sciences "C"

Juan Carlos Bravata-Alcantará, Hospital Juárez de México, Ciudad de México, México

Analyst chemist

Oscar Sosa-Hernández, Hospital General de Zona 2 Benigno Arriaga, Tequisquiapan, San Luis Potosí, México

Epidemiologist

Laura Delgado-Balbuena, Hospital Juárez de México, Ciudad de México, México

Posdoctoral researcher

Gregorio León-García, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México

Researcher

Iliana Alejandra Cortés-Ortíz, Hospital Juárez de México, Ciudad de México, México

Analyst chemist

Monica Alethia Cureño-Díaz, Hospital Juárez de México, Ciudad de México, México

Strategic planning director

Graciela Castro-Escarpulli, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Ciudad de México, México

Researcher

Juan Manuel Vélez-Reséndiz, Escuela Superior de Medicina, Instituto Politécnico Nacional, Ciudad de México, México

Researcher

Juan Manuel Bello-López, Hospital Juárez de México, Ciudad de México, México

Research Deparment

Published
2021-01-31
How to Cite
1.
Durán-Manuel EM, Cruz-Cruz C, Ibáñez-Cervantes G, Bravata-Alcantará JC, Sosa-Hernández O, Delgado-Balbuena L, León-García G, Cortés-Ortíz IA, Cureño-Díaz MA, Castro-Escarpulli G, Vélez-Reséndiz JM, Bello-López JM (2021) Clonal dispersion of Acinetobacter baumannii in an intensive care unit designed to patients COVID-19. J Infect Dev Ctries 15:58-68. doi: 10.3855/jidc.13545
Section
Coronavirus Pandemic