Investigation of the role of stray cats for transmission of toxoplasmosis to humans and animals living in İzmir, Turkey

  • Muhammet Karakavuk Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey
  • Hüseyin Can Department of Biology, Molecular Biology Section, Ege University Faculty of Science, Bornova, İzmir, Turkey
  • Nebahat Selim Veterinary Affairs Directorate, Municipality of Konak, İzmir, Turkey
  • Berna Yeşilsiraz Veterinary Affairs Directorate, Municipality of Narlıdere, İzmir, Turkey
  • Evren Atlı Veterinary Affairs Directorate, Municipality of Narlıdere, İzmir, Turkey
  • Esra Atalay Şahar Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey
  • Ferda Demir Department of Biomedical Technologies, Ege University Faculty of Science, Bornova, İzmir, Turkey
  • Aytül Gül Department of Bioengineering, Ege University Faculty of Engineering, Bornova/İzmir, Turkey
  • Hüseyin Gökhan Özdemir The Protection and Development Union of İzmir Bird Paradise, Konak, İzmir, Turkey
  • Nuray Alan Veterinary Affairs Directorate, Municipality of Çiğli, İzmir, Turkey
  • Mustafa Yalçın Veterinary Affairs Directorate, Municipality of Çiğli, İzmir, Turkey
  • Onur Özkurt Veterinary Affairs Directorate, Municipality of Karabağlar, İzmir, Turkey
  • Murat Aras Veterinary Affairs Directorate, Municipality of Karabağlar, İzmir, Turkey
  • Tuncel Çelik Veterinary Affairs Directorate, Municipality of Karşıyaka, İzmir, Turkey
  • Şengül Can Research Entrepreneurship and Innovation Coordination Center, Manisa Celal Bayar University, Yunusemre, Manisa, Turkey
  • Aysu Değirmenci Döşkaya Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey
  • Adnan Yüksel Gürüz Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey
  • Mert Döşkaya Department of Parasitology, Ege University Faculty of Medicine, Bornova, İzmir, Turkey
Keywords: Toxoplasma gondii, cat, feline, feces, PCR, Turkey

Abstract

Introduction: Toxoplasma gondii is a protozoan parasite that has a widespread distribution among mammalians and birds. One of the reasons for the high prevalence may be due to ingesting oocyst disseminated by stray cats’ feces. In Turkey, most of the citizens are closely associated with stray cats and they love to pet and feed them on the streets. In this study, we aimed to determine the prevalence of T. gondii DNA in feces of stray cats living in İzmir, Turkey in order to identify the transmission potential to humans and other animals.

Methodology: Feces and blood samples of 465 stray cats were investigated for the presence of T. gondii oocysts by microscopy and for the presence of T. gondii DNA by two real time PCR methods. Furthermore, serum samples were analyzed for anti-T. gondii IgG antibodies using an ELISA.

Results: Oocysts were detected in 0.43% of the stray cats by microscopy. T. gondii DNA was detected in 14.37% of the stray cats’ feces samples. The seroprevalence rate was 37.84%. In the feces and/or blood PCR positive group, 35.89% of them were seropositive. Among the 176 seropositive cats, T. gondii DNA was detected in feces of 27 cats (15.34%).

Conclusions: This study first time showed the inter relation of T. gondii DNA in feces and blood samples and seropositivity. In sum, over 14% of the stray cats living outdoor may have an important role in transmission of toxoplasmosis to humans in İzmir as well as to other animals.

Published
2021-01-31
How to Cite
1.
Karakavuk M, Can H, Selim N, Yeşilsiraz B, Atlı E, Atalay Şahar E, Demir F, Gül A, Özdemir HG, Alan N, Yalçın M, Özkurt O, Aras M, Çelik T, Can Şengül, Değirmenci Döşkaya A, Gürüz AY, Döşkaya M (2021) Investigation of the role of stray cats for transmission of toxoplasmosis to humans and animals living in İzmir, Turkey. J Infect Dev Ctries 15:155-162. doi: 10.3855/jidc.13932
Section
Original Articles